Jiang Zhehao, Wang Yuting, Li Wenxiang, Wang Yudan, Liu Xiaojuan, Ou Xi, Su Wei, Song Shiwei, Chen Riyuan
College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
Int J Mol Sci. 2023 Jul 25;24(15):11898. doi: 10.3390/ijms241511898.
Flowering Chinese cabbage ( L. ssp. var. Tsen et Lee) is a widely consumed vegetable in southern China with significant economic value. Developing product organs in the flowering Chinese cabbage involves two key processes: bolting and flowering. Nuclear factor Y (NF-Y) is a heterotrimeric transcription factor known for its crucial role in various plant developmental processes. However, there is limited information available on the involvement of this gene family during flowering during Chinese cabbage development. In this study, 49 genes were identified and characterized along with their physicochemical properties, gene structure, chromosomal location, collinearity, and expression patterns. We also conducted subcellular localization, yeast two-hybrid, and transcriptional activity assays on selected genes. The findings of this study revealed enhanced expression levels of specific genes during the stalk development and flowering stages in flowering Chinese cabbage. Notably, BcNF-YA8, BcNF-YB14, BcNF-YB20, and BcNF-YC5 interacted with BcRGA1, a negative regulator of GA signaling, indicating their potential involvement in GA-mediated stalk development. This study provides valuable insights into the role of genes in flowering Chinese cabbage development and suggests that they are potential candidates for further investigating the key regulators of cabbage bolting and flowering.
菜心（L. ssp. var. Tsen et Lee）是中国南方广泛食用的蔬菜，具有重要的经济价值。菜心产品器官的发育涉及两个关键过程：抽薹和开花。核因子Y（NF-Y）是一种异源三聚体转录因子，因其在各种植物发育过程中的关键作用而闻名。然而，关于该基因家族在菜心发育开花过程中的作用，目前可用信息有限。在本研究中，鉴定并表征了49个基因，包括它们的理化性质、基因结构、染色体定位、共线性和表达模式。我们还对选定的基因进行了亚细胞定位、酵母双杂交和转录活性分析。本研究结果表明，在菜心的茎发育和开花阶段，特定基因的表达水平有所提高。值得注意的是，BcNF-YA8、BcNF-YB14、BcNF-YB20和BcNF-YC5与GA信号的负调节因子BcRGA1相互作用，表明它们可能参与GA介导的茎发育。本研究为基因在菜心发育中的作用提供了有价值的见解，并表明它们是进一步研究白菜抽薹和开花关键调节因子的潜在候选基因。
