Hughes N R, Walls R S, Newland R C, Payne J E
Cancer Res. 1986 Nov;46(11):5993-9.
Two of three monoclonal antibodies to tissue-specific antigens of isolated colonic glands revealed gland to gland heterogeneity of antigen expression in sections of the histologically normal colonic mucosa from patients with colorectal adenocarcinoma or various nonmalignant conditions. A colon-specific goblet cell antigen (designated 3NM) was absent from rare, solitary glands randomly distributed among the otherwise strongly stained glands of distal colon. These 3NM-negative glands stained normally for the other two antigens studied and appeared morphologically and histochemically normal. They occurred in 11 of 13 cancer patients and in each of the five patients with benign conditions with median incidences of 2.2 and 0.4 per 1000 glands, respectively. Gland heterogeneity was also demonstrated in both patient groups for a cell membrane antigen (designated 6NM). In cecum and ascending colon, glands staining strongly and weakly for 6NM were found intermixed. The weakly stained glands tended to predominate in cecum and first part of ascending colon, but they were completely replaced by strongly stained glands in more distal colon. The heterogeneity shown by both 3NM and 6NM appeared due to phenotypically distinct cell clones. Our observations indicate that histologically normal colonic mucosa contains antigenically diverse gland populations.
针对分离出的结肠腺组织特异性抗原的三种单克隆抗体中的两种，在患有结肠直肠癌或各种非恶性疾病患者的组织学正常结肠黏膜切片中，显示出抗原表达的腺间异质性。一种结肠特异性杯状细胞抗原（命名为3NM）在远端结肠其他染色强烈的腺体中随机分布的罕见孤立腺体中缺失。这些3NM阴性腺体对所研究的其他两种抗原染色正常，在形态和组织化学上看起来正常。它们出现在13名癌症患者中的11名以及5名良性疾病患者的每一名中，在癌症患者和良性疾病患者中每1000个腺体的发生率中位数分别为2.2和0.4。在两个患者组中，一种细胞膜抗原（命名为6NM）也表现出腺异质性。在盲肠和升结肠中，发现6NM染色强和弱的腺体相互混杂。染色弱的腺体在盲肠和升结肠起始部占主导，但在更远端的结肠中它们完全被染色强的腺体取代。3NM和6NM显示出的异质性似乎是由于表型不同的细胞克隆所致。我们的观察表明，组织学正常的结肠黏膜包含抗原性多样的腺群体。
