Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology, 70013 Heraklion, Greece.
Department of Industrial Design and Production Engineering, University of West Attica, 12244 Athens, Greece.
Int J Mol Sci. 2023 Aug 3;24(15):12384. doi: 10.3390/ijms241512384.
Circulating cell-free DNA (ccfDNA) is a liquid biopsy biomaterial attracting significant attention for the implementation of precision medicine diagnostics. Deeper knowledge related to its structure and biology would enable the development of such applications. In this study, we employed Raman spectroscopy to unravel the biomolecular profile of human ccfDNA in health and disease. We established reference Raman spectra of ccfDNA samples from healthy males and females with different conditions, including cancer and diabetes, extracting information about their chemical composition. Comparative observations showed a distinct spectral pattern in ccfDNA from breast cancer patients taking neoadjuvant therapy. Raman analysis of ccfDNA from healthy, prediabetic, and diabetic males uncovered some differences in their biomolecular fingerprints. We also studied ccfDNA released from human benign and cancer cell lines and compared it to their respective gDNA, confirming it mirrors its cellular origin. Overall, we explored for the first time Raman spectroscopy in the study of ccfDNA and provided spectra of samples from different sources. Our findings introduce Raman spectroscopy as a new approach to implementing liquid biopsy diagnostics worthy of further elaboration.
循环游离 DNA（ccfDNA）是一种液体活检生物材料，因其在精准医疗诊断中的应用而受到广泛关注。对其结构和生物学的更深入了解将能够推动这些应用的发展。在这项研究中，我们采用拉曼光谱技术揭示了健康和疾病状态下人类 ccfDNA 的生物分子特征。我们建立了来自健康男性和女性（包括癌症和糖尿病患者）的 ccfDNA 样本的参考拉曼光谱，提取了有关其化学成分的信息。比较观察表明，接受新辅助治疗的乳腺癌患者的 ccfDNA 具有明显不同的光谱模式。对健康、糖尿病前期和糖尿病男性的 ccfDNA 的拉曼分析揭示了它们生物分子指纹之间的一些差异。我们还研究了来自人良性和癌细胞系的 ccfDNA，并将其与各自的 gDNA 进行了比较，证实其反映了其细胞来源。总的来说，我们首次在 ccfDNA 的研究中探索了拉曼光谱，并提供了来自不同来源的样本的光谱。我们的研究结果表明，拉曼光谱技术是一种有前途的液体活检诊断方法，值得进一步研究。
