College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an 271018, China.
Int J Mol Sci. 2023 Aug 4;24(15):12447. doi: 10.3390/ijms241512447.
The AT-hook motif nuclear localized () gene family is a highly conserved transcription factor critical for the growth, development, and stress tolerance of plants. However, the function of the gene family in () remains unclear. In this study, 42 family members were identified from the genome and mapped to nine chromosomes. Two clades have formed in the evolution of the gene family. The results showed that most products encoded by family genes are located in the nucleus. Gene duplication was common and expanded the gene family. According to the analysis of -regulatory elements, the genes interact with stress responses (osmotic, cold, and heavy metal stress), major hormones (abscisic acid), and light responses. In addition, the expression profiles revealed that genes are widely expressed in different tissues. was upregulated at 4 h under drought stress, highly expressed under cadmium conditions, and downregulated in response to cold conditions. and were upregulated at the initial time point and peaked at 12 h under cold and cadmium stress, respectively. Notably, the interactions between genes and proteins under drought, cold, and heavy metal stresses were observed when predicting the protein-protein interaction network.
AT-hook motif 核定位()基因家族是一种高度保守的转录因子,对植物的生长、发育和应激耐受至关重要。然而,其在()中的功能尚不清楚。本研究从()基因组中鉴定出 42 个家族成员,并将其映射到 9 条染色体上。在 基因家族的进化过程中形成了两个分支。结果表明,家族基因编码的大多数产物都位于细胞核内。基因复制很常见,从而扩展了 基因家族。根据对-调控元件的分析,这些基因与应激反应(渗透、冷和重金属胁迫)、主要激素(脱落酸)和光反应相互作用。此外,表达谱分析表明,基因在不同组织中广泛表达。在干旱胁迫下,4 h 时上调表达,在镉条件下高表达,在冷胁迫下下调表达。和在冷和镉胁迫下分别在初始时间点上调,并在 12 h 时达到峰值。值得注意的是,在预测蛋白质-蛋白质相互作用网络时,观察到干旱、冷和重金属胁迫下 基因与蛋白质之间的相互作用。