Chen Weifang, Chen Leifu, Cui Lei, Liu Zhixiong, Yuan Weiling
Hubei Key Laboratory of Vegetable Germplasm Innovation and Genetic Improvement, Institute of Economic Crops, Hubei Academy of Agricultural Sciences, Wuhan, China.
Front Plant Sci. 2024 May 30;15:1401414. doi: 10.3389/fpls.2024.1401414. eCollection 2024.
The () gene family is a highly conserved transcription factors involved in plant growth, development, and stress responses. However, have not been systematically analyzed in radish (). Therefore, we performed genome-wide identification and expression pattern, gene structure, and function verifications of radish . We identified 52 radish (), which were unevenly distributed across nine chromosomes. Phylogenetic analysis showed that the were divided into two clades (A and B) and subdivided into three types (I, II, and III). Collinearity analysis revealed that the 52 produced 49 repeat events. Tissue expression profiles revealed differential expression of across different tissues, with higher expression observed in flower organs, particularly petals and anthers. qRT-PCR results indicated that responded to abscisic acid, methyl jasmonate, and abiotic stress (low and high temperatures and drought). Additionally, induced a dwarf phenotype in tomato plants, and -overexpression tomato plants presented significantly decreased expression levels of the gibberellin (GA) synthetic genes , , and , but significantly increased expression of the degradation gene . Thus, might affect plant growth by regulating GA content. Collectively, our study comprehensively identified in radish and provided a reference for further research on these genes.
（）基因家族是参与植物生长、发育和应激反应的高度保守的转录因子。然而，尚未在萝卜（）中进行系统分析。因此，我们对萝卜的进行了全基因组鉴定、表达模式、基因结构和功能验证。我们鉴定出52个萝卜（），它们不均匀地分布在9条染色体上。系统发育分析表明，被分为两个进化枝（A和B）并细分为三种类型（I、II和III）。共线性分析显示，52个产生了49个重复事件。组织表达谱揭示了在不同组织中的差异表达，在花器官，特别是花瓣和花药中观察到更高的表达。qRT-PCR结果表明，对脱落酸、茉莉酸甲酯和非生物胁迫（低温和高温以及干旱）有反应。此外，在番茄植株中诱导了矮化表型，过表达番茄植株中赤霉素（GA）合成基因、和的表达水平显著降低，但降解基因的表达显著增加。因此，可能通过调节GA含量影响植物生长。总体而言，我们的研究全面鉴定了萝卜中的，并为这些基因的进一步研究提供了参考。
