Chen Yong, Gong Youping, Shan Lijun, Tan Chou Yong, Al-Furjan M S, Ramesh S, Chen Huipeng, Bian Xiangjuan, Chen Yanda, Liu Yunfeng, Zhou Rougang
School of Mechanical Engineering, Hangzhou Dianzi University, Hangzhou 310018, China.
State Key Laboratory of Fluid Power and Mechatronic Systems, School of Mechanical Engineering, Zhejiang University, Hangzhou 310027, China.
Materials (Basel). 2023 Jul 28;16(15):5312. doi: 10.3390/ma16155312.
Cartilage damage is difficult to heal and poses a serious problem to human health as it can lead to osteoarthritis. In this work, we explore the application of biological 3D printing to manufacture new cartilage scaffolds to promote cartilage regeneration. The hydrogel made by mixing sodium alginate (SA) and gelatin (GA) has high biocompatibility, but its mechanical properties are poor. The addition of hydroxyapatite (HA) can enhance its mechanical properties. In this paper, the preparation scheme of the SA-GA-HA composite hydrogel cartilage scaffold was explored, the scaffolds prepared with different concentrations were compared, and better formulations were obtained for printing and testing. Mathematical modeling of the printing process of the bracket, simulation analysis of the printing process based on the mathematical model, and adjustment of actual printing parameters based on the results of the simulation were performed. The cartilage scaffold, which was printed using Bioplotter 3D printer, exhibited useful mechanical properties suitable for practical needs. In addition, ATDC-5 cells were seeded on the cartilage scaffolds and the cell survival rate was found to be higher after one week. The findings demonstrated that the fabricated chondrocyte scaffolds had better mechanical properties and biocompatibility, providing a new scaffold strategy for cartilage tissue regeneration.
软骨损伤难以愈合，会给人类健康带来严重问题，因为它可能导致骨关节炎。在这项工作中，我们探索了生物3D打印在制造新型软骨支架以促进软骨再生方面的应用。通过混合海藻酸钠（SA）和明胶（GA）制成的水凝胶具有高生物相容性，但其力学性能较差。添加羟基磷灰石（HA）可以增强其力学性能。本文探索了SA-GA-HA复合水凝胶软骨支架的制备方案，比较了不同浓度制备的支架，并获得了更好的配方用于打印和测试。对支架的打印过程进行数学建模，基于数学模型对打印过程进行模拟分析，并根据模拟结果调整实际打印参数。使用Bioplotter 3D打印机打印的软骨支架表现出适合实际需求的有用力学性能。此外，将ATDC-5细胞接种在软骨支架上，发现一周后细胞存活率更高。这些发现表明，制造的软骨细胞支架具有更好的力学性能和生物相容性，为软骨组织再生提供了一种新的支架策略。
