Sanca Fernando M M, Blanco Iago R, Dias Meriellen, Moreno Andrea M, Martins Simone M M K, Stephano Marco A, Mendes Maria A, Mendonça Carlos M N, Pereira Wellison A, Azevedo Pamela O S, Gierus Martin, Oliveira Ricardo P S
Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, São Paulo 05508-900, Brazil.
Dempster Mass Spectrometry Laboratory, Chemical Engineering Department, Polytechnic School, University of São Paulo, São Paulo 05508-000, Brazil.
Animals (Basel). 2023 Jul 28;13(15):2442. doi: 10.3390/ani13152442.
Swine production is of great importance worldwide and has huge economic and commercial impact. Due to problems with bacterial infection, the use of antimicrobials has increased in the last decades, particularly in Latin America and Asia. This has led to concerns about antimicrobial resistance, which poses risks to human health and the environment. The use of probiotic organisms has been proposed as an alternative to this use, as these beneficial bacteria can produce antimicrobial peptides, such as bacteriocins, which allow the induction of inhibitory effects against pathogenic microorganisms. Among probiotics, some bacteria stand out with the inhibition of animal pathogens. The bacteriocin-like inhibitory substances (BLISs) of subsp. strain L2, present in its cell-free supernatant, were tested against pathogenic strains isolated from pig samples, such as , , , , , and . Compounds secreted by L2 have been shown to inhibit the growth of some pathogenic species, particularly Gram-positive bacteria, with being the most prominent. Antimicrobial peptides with a molecular size of 500-1160 Daltons were isolated from BLISs. The results highlight the potential of BLISs and its peptides as natural antimicrobials for use in the food industry and to reduce the use of growth promoters in animal production.
养猪业在全球范围内具有重要意义，并且具有巨大的经济和商业影响。由于细菌感染问题，在过去几十年中抗菌药物的使用有所增加，特别是在拉丁美洲和亚洲。这引发了对抗菌药物耐药性的担忧，抗菌药物耐药性对人类健康和环境构成风险。有人提议使用益生菌作为抗菌药物使用的替代方法，因为这些有益细菌可以产生抗菌肽，如细菌素，从而对致病微生物产生抑制作用。在益生菌中，一些细菌对动物病原体具有抑制作用，表现突出。对猪亚种L2菌株无细胞上清液中存在的类细菌素抑制物质（BLISs）进行了测试，以对抗从猪样本中分离出的致病菌株，如、、、、和。已证明L2分泌的化合物可抑制某些致病物种的生长，特别是革兰氏阳性菌，其中最为突出。从BLISs中分离出了分子大小为500 - 1160道尔顿的抗菌肽。结果突出了L2 BLISs及其肽作为天然抗菌剂在食品工业中的应用潜力，以及减少动物生产中生长促进剂使用的潜力。
