Gott J M, Shub D A, Belfort M
Cell. 1986 Oct 10;47(1):81-7. doi: 10.1016/0092-8674(86)90368-5.
RNA from T4-infected cells yielded multiple end-labeled species when incubated with alpha-32P-GTP under self-splicing conditions. One of these corresponds to the previously identified intron from the td gene of T4, while others appear to represent additional group I introns in T4. Two loci distinct from the td gene were found to hybridize to a mixed alpha-32P-GTP-labeled T4 RNA probe. These mapped in or near the unlinked genes nrdB and nrdC. A fragment from the nrdB region that contains the intron has been cloned and shown to generate characteristic group I splice products with RNA synthesized in vivo and in vitro. Multiple introns, and the prospect that these occur within several genes in the same metabolic pathway, suggest a possible regulatory role for splicing in T4.
在自我剪接条件下，将来自T4感染细胞的RNA与α-32P-GTP一起孵育时，会产生多个末端标记的物种。其中一个对应于先前鉴定出的T4 td基因中的内含子，而其他的似乎代表T4中的其他I类内含子。发现两个与td基因不同的位点与混合的α-32P-GTP标记的T4 RNA探针杂交。这些位点定位于未连锁的基因nrdB和nrdC中或附近。包含内含子的nrdB区域的一个片段已被克隆，并显示与体内和体外合成的RNA产生特征性的I类剪接产物。多个内含子，以及它们出现在同一代谢途径的几个基因中的可能性，表明剪接在T4中可能具有调节作用。
