Baroni Simona, Argenziano Monica, La Cava Francesca, Soster Marco, Garello Francesca, Lembo David, Cavalli Roberta, Terreno Enzo
Molecular and Preclinical Imaging Centers, Department of Molecular Biotechnology and Health Sciences, University of Torino, Via Nizza 52, 10126 Torino, Italy.
Department of Drug Science and Technology, University of Torino, Via P. Giuria 9, 10125 Torino, Italy.
Nanomaterials (Basel). 2023 Aug 1;13(15):2227. doi: 10.3390/nano13152227.
This paper describes a novel nanoformulation for dual MRI/US in vivo monitoring of drug delivery/release. The nanosystem was made of a perfluoropentane core coated with phospholipids stabilized by glycol chitosan crosslinked with triphosphate ions, and it was co-loaded with the prodrug prednisolone phosphate (PLP) and the structurally similar MRI agent Gd-DTPAMA-CHOL. Importantly, the in vitro release of PLP and Gd-DTPAMA-CHOL from the nanocarrier showed similar profiles, validating the potential impact of the MRI agent as an imaging reporter for the drug release. On the other hand, the nanobubbles were also detectable by US imaging both in vitro and in vivo. Therefore, the temporal evolution of both MRI and US contrast after the administration of the proposed nanosystem could report on the delivery and the release kinetics of the transported drug in a given lesion.
本文描述了一种用于药物递送/释放的双功能磁共振成像（MRI）/超声（US）体内监测的新型纳米制剂。该纳米系统由全氟戊烷核组成，表面包裹有通过与三磷酸离子交联的乙二醇壳聚糖稳定的磷脂，并且它同时负载了前药磷酸泼尼松龙（PLP）和结构相似的MRI剂钆-二乙三胺五乙酸胆酰胺（Gd-DTPAMA-CHOL）。重要的是，PLP和Gd-DTPAMA-CHOL从纳米载体的体外释放显示出相似的曲线，验证了MRI剂作为药物释放成像报告分子的潜在影响。另一方面，纳米气泡在体外和体内均可通过超声成像检测到。因此，在所提出的纳米系统给药后，MRI和超声造影剂的时间演变可以报告给定病变中转运药物的递送和释放动力学。
