微胶囊化乳清分离蛋白-辛烯基琥珀酸酐-淀粉复合凝聚体提高益生菌的存活率。

Improved Viability of Probiotics via Microencapsulation in Whey-Protein-Isolate-Octenyl-Succinic-Anhydride-Starch-Complex Coacervates.

机构信息

Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, School of Food and Bioengineering, Xihua University, Chengdu 610039, China.

Key Laboratory of Grain and Oil Processing and Food Safety of Sichuan Province, College of Food and Bioengineering, Xihua University, Chengdu 610039, China.

出版信息

Molecules. 2023 Jul 28;28(15):5732. doi: 10.3390/molecules28155732.

The aim of this study was to microencapsulate probiotic bacteria ( 11073) using whey-protein-isolate (WPI)-octenyl-succinic-anhydride-starch (OSA-starch)-complex coacervates and to investigate the effects on probiotic bacterial viability during spray drying, simulated gastrointestinal digestion, thermal treatment and long-term storage. The optimum mixing ratio and pH for the preparation of WPI-OSA-starch-complex coacervates were determined to be 2:1 and 4.0, respectively. The combination of WPI and OSA starch under these conditions produced microcapsules with smoother surfaces and more compact structures than WPI-OSA starch alone, due to the electrostatic attraction between WPI and OSA starch. As a result, WPI-OSA-starch microcapsules showed significantly ( < 0.05) higher viability (95.94 ± 1.64%) after spray drying and significantly ( < 0.05) better protection during simulated gastrointestinal digestion, heating (65 °C/30 min and 75 °C/10 min) and storage (4/25 °C for 12 weeks) than WPI-OSA-starch microcapsules. These results demonstrated that WPI-OSA-starch-complex coacervates have excellent potential as a novel wall material for probiotic microencapsulation.

本研究的目的是使用乳清蛋白分离物（WPI）-辛烯琥珀酸酐淀粉（OSA-淀粉）-复合凝聚体微囊化益生菌（11073），并研究喷雾干燥、模拟胃肠道消化、热处理和长期储存过程中对益生菌活菌数的影响。确定 WPI-OSA-淀粉复合凝聚体制备的最佳混合比和 pH 值分别为 2:1 和 4.0。在这些条件下，WPI 和 OSA 淀粉的结合产生了表面更光滑、结构更紧凑的微胶囊，这是由于 WPI 和 OSA 淀粉之间的静电吸引。结果，WPI-OSA-淀粉微胶囊在喷雾干燥后表现出显著更高的活菌数（95.94±1.64%）（<0.05），并且在模拟胃肠道消化、加热（65°C/30min 和 75°C/10min）和储存（4/25°C 12 周）过程中具有更好的保护作用（<0.05），优于 WPI-OSA-淀粉微胶囊。这些结果表明，WPI-OSA-淀粉复合凝聚体具有作为益生菌微囊化新型壁材的巨大潜力。