Department of Chemistry, "Sapienza" University of Rome, p.le A.Moro 5, 00185 Rome, Italy.
Department of Biology and Biotechnologies "C. Darwin", Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Roma, Italy.
Molecules. 2023 Jan 15;28(2):860. doi: 10.3390/molecules28020860.
Recently, there has been a growing interest in producing functional foods containing encapsulated probiotic bacteria due to their positive effects on human health. According to their perceived health benefits, probiotics have been incorporated into a range of dairy products, but the current major challenge is to market new, multicomponent probiotic foods and supplements. Nevertheless, only a few products containing encapsulated probiotic cells can be found as non-refrigerated products. In this work, spray drying technology was investigated in order to produce an innovative nutraceutical formulation based on lactic acid bacteria (LAB), and was able to ensure a good storage stability of probiotics (no less than 109 CFU/cps) in non-refrigerated conditions. Probiotic-loaded microparticles from spray drying experiments were produced under different conditions and compared by thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and the enumeration of the number of viable cells in order to identify the formulation exhibiting the most promising characteristics. Results from the dissolution test revealed that the optimized formulation provides a suitable amount of living cells after digestion of microparticles stored for 12 months at room temperature and confirmed that the microencapsulation process by spray drying ensures a good protection of probiotics for nutraceutical purposes.
最近，由于益生菌对人体健康的积极影响，人们对生产含有封装益生菌的功能性食品越来越感兴趣。根据其被认为的健康益处，益生菌已被纳入一系列乳制品中，但目前的主要挑战是销售新的、多成分益生菌食品和补充剂。然而，只有少数含有封装益生菌细胞的产品可以作为非冷藏产品找到。在这项工作中，研究了喷雾干燥技术，以便生产一种基于乳酸菌（LAB）的创新营养配方，并且能够确保益生菌在非冷藏条件下具有良好的储存稳定性（不少于 109 CFU/cps）。通过喷雾干燥实验生产了负载益生菌的微球，并通过热重分析（TGA）、扫描电子显微镜（SEM）和活菌计数对其进行了比较，以确定具有最有前途特性的配方。溶解试验的结果表明，在室温下储存 12 个月后，优化配方在消化微球后提供了适量的活菌，并且证实喷雾干燥的微囊化过程确保了益生菌在营养方面的良好保护。
