Residual Agrochemical Assessment Division, Department of Agro-Food Safety and Crop Protection, National Institute of Agricultural Sciences, Wanju 55365, Republic of Korea.
Department of Environmental and Biological Chemistry, College of Agriculture, Life and Environment Science, Chungbuk National University, Cheongju 28644, Republic of Korea.
Molecules. 2023 Jul 31;28(15):5774. doi: 10.3390/molecules28155774.
This study investigated the effect of milling on the yields of incurred residues extracted from cereals. Rice, wheat, barley, and oat were soaked in nine pesticides (acetamiprid, azoxystrobin, imidacloprid, ferimzone, etofenprox, tebufenozide, clothianidin, hexaconazole, and indoxacarb), dried, milled, and passed through sieves of various sizes. The quick, easy, cheap, effective, rugged, and safe method and liquid chromatography-tandem mass spectrometry extracted and quantified the incurred pesticides, respectively. For rice and oat, the yields were higher for vortexed samples than for soaked samples. For rice, the yields improved as the extraction time increased from 1 to 5 min. The optimized method was validated based on the selectivity, limit of quantitation, linearity, accuracy, precision, and the matrix effect. For rice and barley, the average yields improved as the particle size decreased from <10 mesh to >60 mesh. For 40-60-mesh wheat and oat, all pesticides (except tebufenozide in oat) had the highest yields. For cereals, 0.5 min vortexing, 5 min extraction, and >40-mesh particle size should be used to optimize incurred pesticide extraction.
本研究考察了粉碎对从谷物中提取的已发生残留量的影响。将水稻、小麦、大麦和燕麦浸泡在九种农药（乙酰甲胺磷、唑菌胺酯、吡虫啉、噻呋酰胺、乙螨唑、虫酰肼、噻虫啉、己唑醇和茚虫威）中，干燥，粉碎并通过不同大小的筛子过筛。分别采用快速、简便、廉价、有效、耐用和安全方法和液相色谱-串联质谱法提取和定量分析了已发生的农药。对于水稻和燕麦，涡旋处理的样品的产率高于浸泡处理的样品。对于水稻，随着提取时间从 1 分钟增加到 5 分钟，产率提高。基于选择性、定量限、线性、准确度、精密度和基质效应，对优化方法进行了验证。对于水稻和大麦，随着粒径从<10 目增加到>60 目，平均产率提高。对于 40-60 目的小麦和燕麦，所有农药（除了燕麦中的虫酰肼）的产率最高。对于谷物，应采用 0.5 分钟涡旋、5 分钟提取和>40 目粒径来优化已发生农药的提取。
