Departamento de Química, Unidad Departamental de Química Analítica, Facultad de Ciencias, Universidad de La Laguna (ULL), Avda. Astrofísico Fco. Sánchez, s/n°, 38206 San Cristóbal de La Laguna, España.
Departamento de Química, Unidad Departamental de Química Analítica, Facultad de Ciencias, Universidad de La Laguna (ULL), Avda. Astrofísico Fco. Sánchez, s/n°, 38206 San Cristóbal de La Laguna, España.
Food Chem. 2023 Jan 30;400:134089. doi: 10.1016/j.foodchem.2022.134089. Epub 2022 Sep 1.
In this work, the development of an analytical method for the evaluation of a wide variety of pesticide residues in cereals and pseudo-cereals widely consumed has been carried out. A QuEChERS method was used as extraction and clean-up procedure prior to separation and quantification of the target analytes by ultra-high performance liquid chromatography coupled to mass spectrometry using a single quadrupole-time-of-flight analyser. The methodology was validated for oat, rye, spelt, barley and quinoa matrices, following the European Commission guidelines (SANTE/11312/2021), achieving good absolute recovery values in the range 60-124% with relative standard deviation values lower than 20% and providing limits of quantification of the method in the low mg/kg range, in accordance with the maximum residue limits established by European policies. Finally, considering the importance of cereals in general population diet, the analysis of 60 samples was carried out in order to assure their safe consumption.
本工作开发了一种分析方法，用于评估广泛食用的谷物和伪谷物中多种农药残留。采用 QuEChERS 方法作为提取和净化程序，然后通过单四极杆-飞行时间分析器将超高效液相色谱与质谱联用，对目标分析物进行分离和定量。该方法按照欧盟委员会指南（SANTE/11312/2021）对燕麦、黑麦、斯佩耳特小麦、大麦和藜麦基质进行了验证，获得了 60-124%的良好绝对回收率，相对标准偏差值低于 20%，并根据欧洲政策规定的最大残留限量，在低 mg/kg 范围内提供了方法的定量限。最后，考虑到谷物在一般人群饮食中的重要性，对 60 个样品进行了分析，以确保其安全食用。
