College of Korean Medicine, Dongshin University, Naju 58245, Republic of Korea.
Molecules. 2023 Aug 3;28(15):5862. doi: 10.3390/molecules28155862.
in Liquamen (BCL), which is extracted from heat-treated fresh bamboo stems, is a traditional herbal medicine widely used in Eastern countries. Recently, it has been reported to have anti-inflammatory and whitening effects. However, the protective effect of BCL on hepatocytes has not yet been elucidated. The present study aimed to determine whether BCL prevents oxidative stress induced by tert-butyl hydroperoxide (t-BHP) and exerts cytoprotective effects on hepatocytes. High-performance liquid chromatography and liquid chromatography with tandem mass spectroscopy were performed to analyze the type of polyphenols present in BCL. The activities of antioxidant enzymes and hepatocyte viability were assessed. The benzoic acid content was the highest among polyphenols present in BCL. Benzoic acid acts as a scavenger of free radicals, including reactive oxygen species. BCL increased the expression of antioxidant enzymes (glutamate-cysteine ligase and NADPH quinone dehydrogenase (1)) by activating nuclear factor erythroid 2-related factor 2 and reduced tBHP-induced cell death by inhibiting oxidative stress. BCL inhibited tBHP-induced phosphorylation of p38 and c-Jun N-terminal kinase but not that of extracellular signal-regulated kinase. In conclusion, BCL is a promising therapeutic candidate for treating oxidative-stress-induced hepatocyte damage.
在 Liquamen (BCL) 中，它是从热处理过的新鲜竹茎中提取的，是一种在东方国家广泛使用的传统草药。最近，它被报道具有抗炎和美白作用。然而，BCL 对肝细胞的保护作用尚未阐明。本研究旨在确定 BCL 是否能预防叔丁基过氧化氢 (t-BHP) 诱导的氧化应激，并对肝细胞发挥细胞保护作用。采用高效液相色谱法和液相色谱-串联质谱法分析 BCL 中多酚的类型。评估抗氧化酶活性和肝细胞活力。在 BCL 中存在的多酚中，苯甲酸含量最高。苯甲酸作为自由基（包括活性氧）的清除剂。BCL 通过激活核因子红细胞 2 相关因子 2 增加抗氧化酶（谷胱甘肽连接酶和 NADPH 醌脱氢酶 (1)) 的表达，并通过抑制氧化应激来减少 tBHP 诱导的细胞死亡。BCL 抑制 tBHP 诱导的 p38 和 c-Jun N-末端激酶的磷酸化，但不抑制细胞外信号调节激酶的磷酸化。总之，BCL 是一种很有前途的治疗氧化应激诱导的肝细胞损伤的治疗候选药物。
