Kubota Riku, Fujimoto Ichiro
Koken Research Institute, Koken Co., Ltd., 1-18-36 Takarada, Tsuruoka-shi, Yamagata 997-0011, Japan.
Polymers (Basel). 2023 Aug 7;15(15):3325. doi: 10.3390/polym15153325.
Preparing strong and flexible atelocollagen-based materials for biomedical applications is still a challenging task. To address this challenge, this study describes the synthesis and characterization of water-soluble polyrotaxanes (PRs) with different coverage ratios and molecular weights of axle polymers, and their potential applications for PR-reinforced atelocollagen threads (PRATs). A novel method was established for the syntheses of PRs with relatively low coverage ratio at the sub-gram scale, in which the aldehyde groups were employed as crosslinking sites for preparing the PRATs via reductive amination. The aldehyde groups were successfully quantified by H nuclear magnetic resonance spectroscopy using 1,1-dimethylhydrazine as an aldehyde marker. Fourier-transform infrared and thermogravimetric analysis measurements supported the characterization of the PRs. Interestingly, tensile testing demonstrated that coverage ratio affected the mechanical properties of the PRATs more strongly than molecular weight. The insights obtained in this study would facilitate the development of soft materials based on atelocollagens and PRs.
制备用于生物医学应用的高强度且柔韧性好的去端肽胶原蛋白基材料仍然是一项具有挑战性的任务。为应对这一挑战，本研究描述了具有不同轴聚合物覆盖率和分子量的水溶性聚轮烷（PRs）的合成与表征，以及它们在PR增强去端肽胶原线（PRATs）中的潜在应用。建立了一种在亚克规模上合成覆盖率相对较低的PRs的新方法，其中醛基被用作通过还原胺化制备PRATs的交联位点。使用1,1 - 二甲基肼作为醛标记物，通过氢核磁共振光谱成功对醛基进行了定量。傅里叶变换红外光谱和热重分析测量结果支持了对PRs的表征。有趣的是，拉伸测试表明，覆盖率对PRATs力学性能的影响比分子量更为显著。本研究获得的见解将有助于基于去端肽胶原蛋白和PRs的软材料的开发。
