一种基于商业试剂盒方法开发的香草扁桃酸（VMA）荧光高效液相色谱测定法。

A fluorometric high performance liquid chromatographic assay for vanillylmandelic acid (VMA) developed from a commercial kit method.

作者信息

Huth J A, Bottjen P L, Ludvigsen C W

出版信息

Clin Biochem. 1986 Aug;19(4):221-4. doi: 10.1016/s0009-9120(86)80030-3.

Abstract

A fluorometric procedure based on the Bio-Rad Vanillylmandelic Acid (VMA) by High Performance Liquid Chromatography (HPLC) Test was developed. Detection by fluorescence provides better sensitivity and specificity than the Pisano spectrophotometric method. Use of a C18 mini-column extraction to extract VMA prior to HPLC analysis significantly reduced the late eluting peaks. A linear range was established up to 400 mumol/L of VMA. Within-run and between-run CV's were 3.0% and 3.7%, respectively. In a comparison of this fluorometric method with the Pisano spectrophotometric method, a linear regression of y = 0.807x + 2.185 was obtained with a correlation coefficient of 0.945. In the analysis of over 50 samples, no intereferences have been found.

摘要

开发了一种基于Bio-Rad高效液相色谱（HPLC）香草扁桃酸（VMA）检测的荧光法。与皮萨诺分光光度法相比，荧光检测具有更高的灵敏度和特异性。在HPLC分析之前，使用C18微型柱萃取来提取VMA，显著减少了后期洗脱峰。建立了高达400μmol/L的VMA线性范围。批内和批间变异系数分别为3.0%和3.7%。在将这种荧光法与皮萨诺分光光度法进行比较时，得到y = 0.807x + 2.185的线性回归，相关系数为0.945。在对50多个样本的分析中，未发现干扰。