Agbaba D, Stojanov M, Rajacic S, Zivanov-Stakic D, Majkic-Singh N
Institute for Medical Biochemistry, University Clinical Center, Belgrade, Serbia.
Clin Chem. 1993 Dec;39(12):2500-3.
We propose a simple and precise densitometric method for measuring vanillylmandelic acid (VMA). The method comprises direct urine application, thin-layer chromatographic (TLC) separation, postchromatographic derivatization, and in situ reading of the formed derivative at 560 nm. Quantitation was done by measuring peak area with a computer-controlled TLC Scanner and applying a five-point calibration function. The calibration function was 7.57-49.23 mumol/L. This method was used to determine urinary concentrations of excreted VMA; concentrations ranged from 4.84 to 56.06 mumol/day.
我们提出了一种简单而精确的光密度法来测定香草扁桃酸（VMA）。该方法包括直接将尿液点样、薄层色谱（TLC）分离、色谱后衍生化以及在560nm处原位读取形成的衍生物。通过使用计算机控制的TLC扫描仪测量峰面积并应用五点校准函数进行定量。校准函数为7.57 - 49.23μmol/L。该方法用于测定排泄的VMA的尿液浓度；浓度范围为4.84至56.06μmol/天。
