Jeppsson J O, Jerntorp P, Sundkvist G, Englund H, Nylund V
Clin Chem. 1986 Oct;32(10):1867-72.
A chromatographic method for determining glycated hemoglobin (Hb A1c) by use of a new monodisperse cation-exchanger has been investigated. Hb A1c was separated from other "minor hemoglobins": Hb F, Hb A3 (the glutathione adduct), and the acetaldehyde adduct in alcoholics. The method was fully automated and a single column could be used for more than 1000 runs. The normal reference interval was 4.0-5.2%; the interval for diabetic outpatients was 5.6-12.4%. Within-run and the between-run CVs were less than 0.9% and 1.7%, respectively. Carbamylation in uremic patients who were undergoing hemodialysis increased the proportion of Hb A1c to 1%. Hb A1c results were compared with results from glucose tolerance tests. In our study, Hb A1c less than 5.5% excluded diabetes: subjects with Hb A1c greater than 6.2% showed diabetes. If blood sampled during fasting had been screened with determinations of glucose and Hb A1c, only 20% of referred subjects would have needed an oral glucose tolerance test for diagnosis of diabetes.
研究了一种使用新型单分散阳离子交换剂测定糖化血红蛋白（Hb A1c）的色谱方法。Hb A1c与其他“次要血红蛋白”分离：Hb F、Hb A3（谷胱甘肽加合物）以及酗酒者中的乙醛加合物。该方法完全自动化，一根柱子可用于1000多次运行。正常参考区间为4.0 - 5.2%；糖尿病门诊患者的区间为5.6 - 12.4%。批内和批间变异系数分别小于0.9%和1.7%。正在接受血液透析的尿毒症患者的氨甲酰化使Hb A1c比例增加至1%。将Hb A1c结果与葡萄糖耐量试验结果进行了比较。在我们的研究中，Hb A1c低于5.5%可排除糖尿病：Hb A1c大于6.2%的受试者患有糖尿病。如果在空腹时采集的血液通过测定血糖和Hb A1c进行筛查，仅20%的转诊受试者需要进行口服葡萄糖耐量试验以诊断糖尿病。
