血红蛋白变体对使用伯乐柱测定糖化血红蛋白的影响。

Effects of hemoglobin variants on Hb A1c determinations using Bio-Rad columns.

作者信息

Allen L C

出版信息

Clin Biochem. 1985 Jun;18(3):173-5. doi: 10.1016/s0009-9120(85)80103-x.

Abstract

In the column chromatographic determination of Hb A1c, hemoglobin variants affect Hb A1c results and column patterns. Samples from several patients with hemoglobin variants were run on Bio-Rad Hb A1c columns to demonstrate these patterns. Columns should be examined during the run to detect the presence of Hb F which migrates very rapidly, and after the run to detect abnormal column patterns. Hb A1c levels in patients heterozygous for Hb S or C are low but may be interpreted in relation to the patient's level of Hb A. On the other hand, Hb A1c results on patients with homozygous Hb S, Hb C, or high levels of Hb F cannot be interpreted.

摘要

在血红蛋白A1c的柱色谱测定中，血红蛋白变体影响血红蛋白A1c结果和柱型图谱。从几名患有血红蛋白变体的患者采集的样本在伯乐血红蛋白A1c柱上进行分析，以展示这些图谱。在分析过程中应检查色谱柱，以检测迁移速度非常快的胎儿血红蛋白（Hb F）的存在，分析结束后检查色谱柱以检测异常的柱型图谱。携带Hb S或Hb C杂合子的患者的血红蛋白A1c水平较低，但可能需要结合患者的血红蛋白A水平进行解读。另一方面，患有纯合Hb S、Hb C或高胎儿血红蛋白水平患者的血红蛋白A1c结果无法进行解读。