Waite K V, Maberly G F, Ma G, Eastman C J
Clin Chem. 1986 Oct;32(10):1966-8.
We adapted a commercial immunoradiometric assay (IRMA) to measure thyrotropin in filter-paper blood spots. Two 3-mm blood spots are used for each standard and sample. These are incubated for 2 h with radiolabeled antibody and for 30 min with magnetic antibody, followed by a 10-min separation procedure. Assay sensitivity is 6 milli-int. units/L. Coefficients of variation (precision profile of the standard curve) ranged from 4.3 to 9.6%. The coefficient of correlation (r) between thyrotropin concentrations in the blood spots and in serum was 0.93. Pre-elution of the blood spots is necessary for short incubation time. Short incubation time, little need for specialized equipment, the high precision and sensitivity characteristic of IRMA, and ease of collection, transport, and storage of the blood-spot samples make this assay suitable for neonatal hypothyroid screening.
我们采用了一种商业化免疫放射分析（IRMA）方法来检测滤纸血斑中的促甲状腺激素。每个标准品和样本使用两个3毫米的血斑。将它们与放射性标记抗体孵育2小时，再与磁性抗体孵育30分钟，随后进行10分钟的分离步骤。检测灵敏度为6毫国际单位/升。变异系数（标准曲线的精密度曲线）范围为4.3%至9.6%。血斑中促甲状腺激素浓度与血清中促甲状腺激素浓度之间的相关系数（r）为0.93。对于较短的孵育时间，血斑预洗脱是必要的。较短的孵育时间、对专业设备需求较少、IRMA的高精度和高灵敏度特性以及血斑样本易于采集、运输和储存，使得该检测方法适用于新生儿甲状腺功能减退筛查。
