Arends J, Nørgaard-Pedersen B
Clin Chem. 1986 Oct;32(10):1854-6.
We have evaluated a time-resolved immunofluorometric assay (IFMA) for determining thyrotropin. This "sandwich"-type system involves two monoclonal antibodies directed against different epitopes. A linear relationship between signal and thyrotropin concentration was observed up to 6000 milli-int. units/L. This procedure takes one day, vs six days with our present RIA technique, and requires only a tenth as much sample. Furthermore, intra- and interassay CVs are lower than with RIA. Assay of 19 paper-disc blood specimens from newborns identified as having congenital hypothyroidism, both by RIA and by clinical evidence, also gave positive results with IFMA. In prospective assay of 3944 specimens by both methods we identified one case of congenital hypothyroidism, which was detected by both techniques. Technical false-positive reactions, identified as such by repeated analyses, were fewer with the IFMA method than with RIA.
我们评估了一种用于测定促甲状腺激素的时间分辨免疫荧光分析（IFMA）方法。这种“夹心”型系统涉及两种针对不同表位的单克隆抗体。在促甲状腺激素浓度高达6000毫国际单位/升时，观察到信号与促甲状腺激素浓度之间呈线性关系。该方法只需一天时间，而我们目前的放射免疫分析（RIA）技术则需要六天，并且所需样本量仅为RIA的十分之一。此外，批内和批间变异系数低于RIA。对19份经RIA和临床证据鉴定为患有先天性甲状腺功能减退症的新生儿滤纸血标本进行检测，IFMA也得出了阳性结果。通过两种方法对3944份标本进行前瞻性检测时，我们发现了一例先天性甲状腺功能减退症病例，两种技术均检测到该病例。经反复分析确定为技术假阳性反应的情况，IFMA方法比RIA方法少。
