Chemistry Department, Ball State University, Muncie, IN, USA.
Methods Mol Biol. 2023;2709:151-161. doi: 10.1007/978-1-0716-3417-2_9.
The advances in nucleic acid nanotechnology have given rise to various elegantly designed structural complexes fabricated from DNA, RNA, chemically modified RNA strands, and their mixtures. The structural properties of NA nanoparticles (NANP) generally dictate and significantly impact biological function; and thus, it is critical to extract information regarding relative stabilities of the different structural forms. The adequate stability assessment requires knowledge of thermodynamic parameters that can be empirically derived using conventional UV-melting technique. The focus of this chapter is to describe methodology to evaluate thermodynamic data of NANPs complexation based on DNA 12 base-pair (bp) duplex formation as an example.
核酸纳米技术的进步催生了各种精心设计的结构复合物，这些复合物由 DNA、RNA、化学修饰的 RNA 链及其混合物制成。核酸纳米颗粒 (NANP) 的结构特性通常决定并显著影响生物功能；因此，提取有关不同结构形式相对稳定性的信息至关重要。充分的稳定性评估需要了解热力学参数，可以使用常规的紫外光融解技术从经验中得出。本章的重点是描述一种评估基于 DNA 12 碱基对 (bp) 双链体形成的 NANP 复合物热力学数据的方法，以 DNA 12 碱基对 (bp) 双链体形成为例。
