小体积微流变学评估基于核酸的超组装体的粘弹性质。
Small Volume Microrheology to Evaluate Viscoelastic Properties of Nucleic Acid-Based Supra-Assemblies.
机构信息
Department of Physics, University of Nebraska Omaha, Omaha, NE, USA.
Nanoscale Science Program, Department of Chemistry, University of North Carolina at Charlotte, Charlotte, NC, USA.
出版信息
Methods Mol Biol. 2023;2709:179-189. doi: 10.1007/978-1-0716-3417-2_11.
Particle tracking (PT) microrheology is a passive microrheological approach that characterizes material properties of soft matter. Multicomponent materials with the ability to create extensive crosslinking, such as supra-assemblies, may exhibit a complex interplay of viscous and elastic properties with a substantial contribution of liquid phase still diffusing through the system. Microrheology analyzes the motion of microscopic beads immersed in a sample, making it possible to evaluate the rheological properties of biological supra-assemblies. This method requires only a small volume of the sample and a relatively simple, inexpensive experimental setup. The objective of this chapter is to describe the experimental procedures for the observation of particle motion, calibration of an optical setup for particle tracking, preparation of imaging chambers, and the use of image analysis software for particle tracking in viscoelastic nucleic acid-based supra-assemblies.
粒子追踪（PT）微流变学是一种被动的微流变方法，用于表征软物质的材料特性。具有形成广泛交联能力的多组分材料，如超组装体，可能表现出粘性和弹性性质的复杂相互作用，并且仍有大量液相在系统中扩散。微流变学分析了浸入样品中的微观珠的运动，从而可以评估生物超组装体的流变性质。该方法仅需要少量的样品，并且实验设置相对简单，成本低廉。本章的目的是描述观察粒子运动的实验程序、粒子跟踪的光学设置校准、成像室的制备以及用于粘性核酸基超组装体中粒子跟踪的图像分析软件的使用。
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