用于核酸纳米颗粒递送的 PAMAM 树枝状大分子的表征。
Characterization of PAMAM Dendrimers for the Delivery of Nucleic Acid Nanoparticles.
机构信息
Nanoscale Science Program, Department of Chemistry, University of North Carolina at Charlotte, Charlotte, NC, USA.
出版信息
Methods Mol Biol. 2023;2709:253-259. doi: 10.1007/978-1-0716-3417-2_17.
Abstract
The protocols described in this book chapter are meant to be used as an outline and guideline to explore the use of a cationic, polymeric, and synthetic carrier-poly (amidoamine) (PAMAM) dendrimers. The amine-terminated, hyperbranched structures have been identified as a vehicle for the delivery of nucleic acids. As such, clear protocols for the optimization of dendrimer usage should be set in place. This chapter details the experiments used to determine the ratio that dendrimers and nucleic acids should be complexed at through the use of binding assays, nuclease protection assays, and competitive binding assays.
摘要
本章中描述的方案旨在用作探索使用阳离子聚合物和合成载体聚（酰胺-胺）（PAMAM）树枝状大分子的大纲和指南。已将胺端基的超支化结构鉴定为输送核酸的载体。因此，应该制定用于优化树枝状大分子使用的明确方案。本章详细介绍了通过使用结合测定法、核酸酶保护测定法和竞争性结合测定法来确定树枝状大分子和核酸复合物的比例的实验。
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