使用比色分割 G-四链体探针检测多重 NASBA RNA 产物。
Detection of Multiplex NASBA RNA Products Using Colorimetric Split G Quadruplex Probes.
机构信息
Laboratory of DNA-Nanosensor Diagnostics, ITMO University, Saint Petersburg, Russia.
Laboratory of Amyloid Biology, Saint-Petersburg State University, Saint Petersburg, Russia.
出版信息
Methods Mol Biol. 2023;2709:287-298. doi: 10.1007/978-1-0716-3417-2_20.
Structural RNA is a challenging target for recognition by hybridization probes. This chapter addresses the recognition problem of RNA amplicons in samples obtained by multiplex nucleic acid sequence-based amplification (NASBA). The method describes the design of G-quadruplex binary (split) DNA peroxidase sensors that produces colorimetric signal upon recognition of NASBA amplicons.
结构 RNA 是杂交探针识别的一个具有挑战性的靶标。本章讨论了通过多重核酸序列扩增（NASBA）获得的样品中 RNA 扩增子的识别问题。该方法描述了 G-四链体二元（分裂）DNA 过氧化物酶传感器的设计，该传感器在识别 NASBA 扩增子时会产生比色信号。
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