College of Chemistry and Material Science, Shandong Agricultural University, Taian 271018, PR China; Key Laboratory of Agricultural Film Application of Ministry of Agriculture and Rural Affairs, Taian 271018, PR China.
College of Chemistry and Material Science, Shandong Agricultural University, Taian 271018, PR China; College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, PR China.
Food Chem. 2024 Jan 15;431:137097. doi: 10.1016/j.foodchem.2023.137097. Epub 2023 Aug 6.
In this work, a rapid, specific, and visual ratiometric fluorescence probe was constructed for tetracycline hydrochloride (TCH) determination based on zinc-doped carbon quantum dots (Zn-CDs). In the presence of TCH, the blue fluorescence at 440 nm originating from Zn-CDs was quenched, and the green fluorescence at 515 nm stemming from TCH was enhanced. The inner filter effect (IFE) and the chelation between Zn and tetracycline are the main mechanisms for the conversion of spectra. The spectrum and color change completed and stabilized within 1 min, indicating the possibility of real-time detection of TCH. The detection range for TCH is 0.1-50 μM, and the low detection limit is 61.1 nM. In addition, Zn-CDs-based test strips were successfully applied to direct visual identification of TCH in actual samples of river water and milk, indicating the possibility of their practical application.
在这项工作中，基于锌掺杂碳量子点（Zn-CDs）构建了一种用于盐酸四环素（TCH）测定的快速、特异、可视化的比率荧光探针。在 TCH 的存在下，源于 Zn-CDs 的 440nm 处的蓝色荧光被猝灭，而源于 TCH 的 515nm 处的绿色荧光增强。内滤效应（IFE）和 Zn 与四环素之间的螯合作用是光谱转换的主要机制。光谱和颜色变化在 1 分钟内完成并稳定下来，表明 TCH 的实时检测成为可能。TCH 的检测范围为 0.1-50μM，检测下限低至 61.1nM。此外，Zn-CDs 基试条成功应用于河流水和牛奶实际样品中 TCH 的直接可视化识别，表明其实际应用的可能性。
