Yu Peilan, Ji Lintong, Wang Teng, Hu Juan, Jiang Lihao, Guo Shancai, Pan Yongjian, Lin Jianyuan
Zhejiang Wanli University, Ningbo, 315100, Zhejiang Province, China.
The Key Laboratory of Quality and Safety Testing and Control of Ningbo Characteristic Agricultural Products, Ningbo, 315100, Zhejiang Province, China.
J Fluoresc. 2024 Dec 16. doi: 10.1007/s10895-024-04080-2.
The detection of tetracycline antibiotics in environmental waters is crucial due to their widespread use, persistence, and potential toxicity. Herein, a method for the specific detection of tetracycline in aquaculture wastewater using a nitrogen-doped carbon quantum dots fluorescence probe is reported. Nitrogen-doped carbon quantum dots (N-CQDs) were synthesized in one step via a hydrothermal method, employing citric acid as the carbon source and diethylenetriamine as the nitrogen source. The resulting N-CQDs exhibit excellent stability, solubility and fluorescence properties. Upon the introduction of tetracycline, a fluorescence burst can be observed, indicating that the N-CQDs function as a ratio fluorescence probe. The fluorescence burst phenomenon is primarily due to the internal filtering effect. In the case of N-CQDs, this burst is attributed to the overlap between the absorption spectrum of tetracycline and the emission spectrum of the carbon quantum dots, which results in internal filtering. The linear range for tetracycline detection spans from 54.2 nM to 0.8 μM, with a detection limit of 54.2 nM. The developed fluorescence probe shows potential for application in detecting tetracycline in real water samples.
由于四环素类抗生素的广泛使用、持久性和潜在毒性，环境水体中四环素类抗生素的检测至关重要。在此，报道了一种使用氮掺杂碳量子点荧光探针特异性检测水产养殖废水中四环素的方法。以柠檬酸为碳源、二亚乙基三胺为氮源，通过水热法一步合成了氮掺杂碳量子点（N-CQDs）。所得的N-CQDs表现出优异的稳定性、溶解性和荧光性能。引入四环素后，可观察到荧光猝发，这表明N-CQDs作为比率荧光探针发挥作用。荧光猝发现象主要归因于内滤效应。对于N-CQDs，这种猝发归因于四环素的吸收光谱与碳量子点的发射光谱之间的重叠，从而导致内滤。四环素检测的线性范围为54.2 nM至0.8 μM，检测限为54.2 nM。所开发的荧光探针在实际水样中检测四环素具有应用潜力。
