Tandon Deepti, Shah Nainisha, Goriwale Mayuri, Karandikar Kalyani, Begum Shahina, Patil Anushree D, Munne Kiran, Kamat Sharmila, Aranha Clara, Bhor Vikrant M
Department of Clinical Research, ICMR-National Institute for Research in Reproductive and Child Health, Mumbai, India.
Department of Molecular Immunology and Microbiology, ICMR- National Institute for Research in Reproductive and Child Health (NIRRCH), Mumbai, Maharashtra, India.
Indian J Med Microbiol. 2023 Sep-Oct;45:100393. doi: 10.1016/j.ijmmb.2023.100393. Epub 2023 Jun 6.
The vaginal microbiome contributes significantly to women's reproductive health and fluctuates due to various physiological and pathological factors. The study's objective is to map the vaginal microbiome of non-pregnant women and evaluate variations based on various potential factors influencing vaginal milieu.
Fifty-two sexually active, non-pregnant women between 18 and 45 years were recruited from a community clinic and clinical history was recorded. Vaginal swabs were collected to assess the vaginal microbiome by sequencing the V3-V4 region of the 16S rRNA using the Illumina HiSeq platform, followed by data analysis with QIIME 2. Vaginal milieu was assessed by Nugent score and profiling cytokines in the cervico-vaginal lavage.
Lactobacillus iners (34.3%) were the most abundant species in all women. Significant changes in abundance of genera (Lactobacillus, Prevotella and Anaerococcus), expression of pro-inflammatory cytokine IFN-γ and changes in alpha and beta diversity was observed in women having asymptomatic bacterial vaginosis (BV). Differences in beta diversity were seen between healthy women and women exhibiting presence of Candida spp. Variations in the abundance of genera (Lactobacillus, Bifidobacterium, Porphyromonas) were observed in women who had delivery less than twelve months back, probably as more of these women (50%, 53.7%) had higher abnormal Nugent score.
Lactobacillus iners was the most prevalent vaginal species in women from a Mumbai community clinic. Maximum variations in the vaginal microbiome characterized by a perturbation of the Lactobacillus predominant vaginal microbiota are seen in those women who have asymptomatic BV and childbirth within last twelve months.
阴道微生物群对女性生殖健康有重要影响，并因各种生理和病理因素而波动。本研究的目的是描绘非孕妇的阴道微生物群，并根据影响阴道环境的各种潜在因素评估其变化。
从社区诊所招募了52名年龄在18至45岁之间、有性生活的非孕妇，并记录其临床病史。采集阴道拭子，使用Illumina HiSeq平台对16S rRNA的V3-V4区域进行测序，以评估阴道微生物群，随后使用QIIME 2进行数据分析。通过Nugent评分和分析宫颈阴道灌洗液中的细胞因子来评估阴道环境。
惰性乳杆菌（34.3%）是所有女性中最丰富的菌种。在无症状细菌性阴道病（BV）患者中，观察到菌属（乳杆菌属、普雷沃菌属和厌氧球菌属）丰度的显著变化、促炎细胞因子IFN-γ的表达以及α和β多样性的变化。健康女性与念珠菌属阳性女性之间观察到β多样性的差异。在分娩不到12个月的女性中，观察到菌属（乳杆菌属、双歧杆菌属、卟啉单胞菌属）丰度的变化，可能是因为这些女性中更多人（50%，53.7%）的Nugent评分异常较高。
惰性乳杆菌是孟买社区诊所女性中最常见的阴道菌种。在过去12个月内有无症状BV和分娩的女性中，以乳杆菌为主的阴道微生物群受到干扰为特征的阴道微生物群变化最大。
