Elangovan Divyaa, Neeravi Ayyanraj, Sahni Rani Diana, Santhanam Sridhar, Beck Manisha Madhai, Adhiya Ranjan, Kwatra Gaurav, Solaimalai Dhanalakshmi, Veeraraghavan Balaji
Department of Clinical Microbiology, Christian Medical College, Vellore, India.
Department of Neonatology, Christian Medical College, Vellore, India.
Indian J Med Microbiol. 2023 Sep-Oct;45:100392. doi: 10.1016/j.ijmmb.2023.100392. Epub 2023 Jun 7.
Invasive group B Streptococcal disease (iGBS) is an important cause of morbidity and mortality in neonates for which the development of an efficacious vaccine remains a global health imperative. The knowledge about the serotype distribution of iGBS is important component for formulation of Capsular polysaccharide (CPS)-based vaccine. However, there were absolute lack of information on serotype distribution in invasive GBS isolates from Indian subcontinent. Methods This study has assessed the serotype distribution and antimicrobial susceptibility profile of invasive group B streptococcal isolates for a period of 13 years from 2009 to 2022 from a tertiary care Center in South India. A total of 155 iGBS isolates were subjected to serotyping by conventional multiplex PCR for identification of all ten GBS serotype. Antimicrobial susceptibility profile and demographic details were extracted from microbiological records. Results Overall, the most common serotype causing invasive GBS were Ia (29%), V (26%), III (15%), II (12%), VI (6%), VII (5%) and Ib (5%). Serotypes IV, VIII and XI were not detected. Among the early-onset iGBS, the common serotype were Ia (36%), V (27%), and III (8%). In late onset iGBS, Serotype III (44%) was predominant. The common serotype in adults were Serotype V (31%) and III (20%). All the invasive GBS isolates were susceptible for penicillin (100%), but the susceptibility for clindamycin and erythromycin were 72% and 80% respectively. Conclusion The serotype distribution of invasive Group B streptococcal isolates from India suggest that hexavalent group B CPS vaccine will cover only 90% of GBS isolates causing invasive disease among the infants in India. Continued surveillance monitoring for serotype distribution and antimicrobial resistance patterns for iGBS are warranted to make public health interventions.
侵袭性B族链球菌病（iGBS）是新生儿发病和死亡的重要原因，开发有效的疫苗仍然是全球卫生的当务之急。了解iGBS的血清型分布是基于荚膜多糖（CPS）疫苗配方的重要组成部分。然而，关于印度次大陆侵袭性GBS分离株的血清型分布完全缺乏相关信息。方法 本研究评估了2009年至2022年期间来自印度南部一家三级护理中心的侵袭性B族链球菌分离株的血清型分布和抗菌药物敏感性概况。共对155株iGBS分离株进行常规多重PCR血清分型，以鉴定所有10种GBS血清型。从微生物学记录中提取抗菌药物敏感性概况和人口统计学细节。结果 总体而言，引起侵袭性GBS的最常见血清型为Ia（29%）、V（26%）、III（15%）、II（12%）、VI（6%）、VII（5%）和Ib（5%）。未检测到血清型IV、VIII和XI。在早发性iGBS中，常见血清型为Ia（36%）、V（27%）和III（8%）。在晚发性iGBS中，血清型III（44%）占主导地位。成人中的常见血清型为血清型V（31%）和III（20%）。所有侵袭性GBS分离株对青霉素均敏感（100%），但对克林霉素和红霉素的敏感性分别为72%和80%。结论 来自印度的侵袭性B族链球菌分离株的血清型分布表明，六价B族CPS疫苗仅能覆盖印度婴儿中90%引起侵袭性疾病的GBS分离株。有必要持续监测iGBS的血清型分布和抗菌药物耐药模式，以便进行公共卫生干预。
