Smallbone B W, Davies B J, Noulty E J, Pisters L L, Taylor N E, McDonald J W
Clin Invest Med. 1986;9(3):145-9.
Infusion of plasma containing zymosan-activated complement into sheep produces leukopenia, pulmonary leukostasis, pulmonary hypertension, hypoxia and increased plasma levels of thromboxane. We investigated the effects of the calcium channel blocking agent verapamil in this system using conscious sheep. Verapamil in 5 mg and 10 mg doses was administered by intravenous infusion prior to an infusion of autologous plasma containing zymosan-activated complement. Pretreatment with verapamil inhibited thromboxane synthesis, the rise in pulmonary artery pressure and the hypoxia without affecting the transient leukopenia. These effects are similar to those previously demonstrated with nonsteroidal antinflammatory drugs, suggesting that verapamil is acting at one or more early steps of the arachidonic acid cascade in addition to its influence on the calcium-sensitive protein interactions involved in smooth muscle function.
将含有酵母聚糖激活补体的血浆输注到绵羊体内会导致白细胞减少、肺白细胞淤滞、肺动脉高压、缺氧以及血浆血栓素水平升高。我们使用清醒的绵羊在该系统中研究了钙通道阻滞剂维拉帕米的作用。在输注含有酵母聚糖激活补体的自体血浆之前，通过静脉输注给予5毫克和10毫克剂量的维拉帕米。维拉帕米预处理可抑制血栓素合成、肺动脉压升高和缺氧，而不影响短暂性白细胞减少。这些作用与先前用非甾体抗炎药所证明的作用相似，表明维拉帕米除了影响参与平滑肌功能的钙敏感蛋白相互作用外，还作用于花生四烯酸级联反应的一个或多个早期步骤。
