School of Resources & Civil Engineering, Northeastern University, Shenyang 110819, China.
School of Resources & Civil Engineering, Northeastern University, Shenyang 110819, China; Centre for Regional Oceans, and Department of Ocean Science and Technology, Faculty of Science and Technology, University of Macau, Macau 999078, China.
Bioresour Technol. 2023 Nov;387:129663. doi: 10.1016/j.biortech.2023.129663. Epub 2023 Aug 12.
The strategy of high reflux ratio and long solids retention time was adopted to realize efficient nitrogen removal from real shale oil wastewater. This was undertaken with a low chemical oxygen demand to total nitrogen (COD/TN) ratio by strengthening aerobic denitrification in an anoxic/aerobic membrane bioreactor (A/O-MBR). The TN removal load climbed from 22 to 25 g N/(kg MLSS·d) as the COD/TN ratio declined from 8 to 3. The abundance of heterotrophic nitrifying and aerobic denitrifying (HNAD) bacteria increased by 13.8 times to 42.5%, displacing anoxic denitrifying bacteria as the predominant bacteria. The abundance of genes involved in denitrification (napAB, narGHI, norBC, nosZ) increased, however the genes related to assimilatory nitrate reduction (nirA, narB, nasC) decreased. The capacity of the dominant HNAD bacteria in an A/O-MBR to efficiently utilize a carbon source is the key to efficient nitrogen removal from shale oil wastewater with a low COD/TN ratio.
采用高回流比和长固体停留时间的策略，通过在缺氧/好氧膜生物反应器（A/O-MBR）中强化好氧反硝化作用，从实际页岩油废水中实现高效脱氮。这是通过在缺氧/好氧膜生物反应器（A/O-MBR）中强化好氧反硝化作用来实现的，其方法是采用低化学需氧量与总氮（COD/TN）比值，以实现高效脱氮。随着 COD/TN 比从 8 降至 3，TN 去除负荷从 22 增加至 25 g N/（kg MLSS·d）。异养硝化和好氧反硝化（HNAD）细菌的丰度增加了 13.8 倍，达到 42.5%，取代了缺氧反硝化细菌成为主要细菌。尽管与同化硝酸盐还原相关的基因（nirA、narB、nasC）减少了，但参与反硝化的基因（napAB、narGHI、norBC、nosZ）增加了。A/O-MBR 中优势 HNAD 细菌有效利用碳源的能力是从低 COD/TN 比值的页岩油废水中高效去除氮的关键。
