Wenzhou Key Laboratory of Sanitary Microbiology, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, China.
Wenzhou Center for Disease Control and Prevention, Wenzhou, China.
Mol Phylogenet Evol. 2023 Nov;188:107903. doi: 10.1016/j.ympev.2023.107903. Epub 2023 Aug 11.
Yersinia spp. vary significantly in their ability to cause diseases that threaten public health. Their pathogenicity is frequently associated with increasing antimicrobial resistance (AMR) and various virulence factors. The aim of the study was to investigate the AMR genes, virulence factors, and genetic diversity of Yersinia strains isolated from meats and fish in Wenzhou in 2020 by using whole-genome sequencing (WGS). A total of 50 isolates were collected. The phylogenetic relationships among the Yersinia species were also analyzed using multilocus sequence typing (MLST), core genome multi-locus sequence typing (cgMLST), and single nucleotide polymorphism (SNP) analysis. According to the results, all the strains could be classified into five species, with most isolated from beef, followed by poultry, pork, and fish. AMR genes were identified in 23 strains. And the qnrD1 genes were all located in the Col3M plasmid. Virulence genes, such as yaxA, ystB, pla, and yplA, were also found in the 15 Y. enterocolitica strains. And this study also found the presence of icm/dot type IVB-related genes in one Yersinia massiliensis isolate. MLST analysis identified 43 sequence types (STs), 19 of which were newly detected in Yersinia. Moreover, cgMLST analysis revealed that no dense genotype clusters were formed (cgMLST 5341, 5344, 5346-5350, 5353-5390). Instead, the strains appeared to be dispersed over large distances, except when multiple isolates shared the same ST. Isolates Y4 and Y26 were closely related to strains originating from South Korea and Denmark. This study showed considerable diversity in Yersinia spp. isolated from local areas (Wenzhou City). The data generated in our study may enrich the molecular traceability database of Yersinia and provide a basis for the development of more effective antipathogen control strategies.
耶尔森氏菌属在引起威胁公共健康的疾病方面的能力差异很大。其致病性常与不断增加的抗微生物药物耐药性（AMR）和各种毒力因子有关。本研究旨在通过全基因组测序（WGS），调查 2020 年从温州肉类和鱼类中分离的耶尔森氏菌的 AMR 基因、毒力因子和遗传多样性。共收集了 50 株分离株。还使用多位点序列分型（MLST）、核心基因组多位点序列分型（cgMLST）和单核苷酸多态性（SNP）分析来分析耶尔森氏菌种之间的系统发育关系。结果表明，所有菌株均可分为五个种，其中大部分分离自牛肉，其次是家禽、猪肉和鱼类。在 23 株菌中鉴定出 AMR 基因。qnrD1 基因均位于 Col3M 质粒上。在 15 株肠型耶尔森氏菌中还发现了 ystB、pla 和 yplA 等毒力基因。本研究还发现一株马塞耶尔森氏菌分离株存在 icm/dot 型 IVB 相关基因。MLST 分析确定了 43 个序列型（ST），其中 19 个是在耶尔森氏菌中首次检测到的。此外，cgMLST 分析表明没有形成密集的基因型簇（cgMLST 5341、5344、5346-5350、5353-5390）。相反，除了多个分离株具有相同的 ST 外，菌株似乎分散在很大的距离上。分离株 Y4 和 Y26 与来自韩国和丹麦的菌株密切相关。本研究表明，从当地（温州市）分离的耶尔森氏菌具有相当大的多样性。本研究产生的数据可能丰富耶尔森氏菌的分子溯源数据库，并为制定更有效的抗病原体控制策略提供依据。
