Institut de Génétique et de Biologie Moléculaire et Cellulaire, 67404 Illkirch, France; Centre National de la Recherche Scientifique, UMR7104, 67404 Illkirch, France; Institut National de la Santé et de la Recherche Médicale, U1258, 67404 Illkirch, France; Université de Strasbourg, 67404 Illkirch, France.
Université de Strasbourg, 67404 Illkirch, France.
Trends Biochem Sci. 2023 Oct;48(10):839-848. doi: 10.1016/j.tibs.2023.07.009. Epub 2023 Aug 12.
Core promoters are sites where transcriptional regulatory inputs of a gene are integrated to direct the assembly of the preinitiation complex (PIC) and RNA polymerase II (Pol II) transcription output. Until now, core promoter functions have been investigated by distinct methods, including Pol II transcription initiation site mappings and structural characterization of PICs on distinct promoters. Here, we bring together these previously unconnected observations and hypothesize how, on metazoan TATA promoters, the precisely structured building up of transcription factor (TF) IID-based PICs results in sharp transcription start site (TSS) selection; or, in contrast, how the less strictly controlled positioning of the TATA-less promoter DNA relative to TFIID-core PIC components results in alternative broad TSS selections by Pol II.
核心启动子是基因转录调控输入整合的位点，用于指导起始复合物（PIC）和 RNA 聚合酶 II（Pol II）转录产物的组装。到目前为止，核心启动子的功能已经通过不同的方法进行了研究，包括 Pol II 转录起始位点作图和不同启动子上 PIC 的结构特征。在这里，我们将这些以前未连接的观察结果结合起来，并假设在真核生物 TATA 启动子上，TFIID 为基础的 PIC 精确结构的构建如何导致转录起始位点（TSS）的选择；或者，相反，TATA 缺失启动子 DNA 相对于 TFIID-核心 PIC 组件的位置控制不严格如何导致 Pol II 进行替代的广泛 TSS 选择。
