Can Gerçek Yusuf, Kutlu Naciye, Çelik Saffet, Gıdık Betül, Bayram Sinan, Bayram Nesrin Ecem
Department of Biology, Faculty of Science, Istanbul University, 34116, Istanbul, Turkey.
Center for Plant and Herbal Products Research-Development, 34134, Istanbul, Turkey.
Chem Biodivers. 2023 Sep;20(9):e202300417. doi: 10.1002/cbdv.202300417. Epub 2023 Sep 4.
In this study, it was aimed to examine the capacity of deep eutectic solvents (DESs) with different contents to extract bioactive compounds from tarragon (Artemisia dracunculus L.) plant. For this reason, the total phenolic-flavonoid content, total proanthocyanidin content and antioxidant/antimicrobial activities of the prepared DES extracts were investigated, as well as the individual phenolic and individual amino acid profiles. According to the results, DES10 had the highest efficiency in terms of its capacity to extract individual phenolics (approximately 59 mg/100 g) and individual amino acids (approximately 2500 mg/kg), and also gave a higher yield compared to ethanol (approximately 44 mg/100 g for individual phenolics and about 19810 mg/kg for individual amino acids) and methanol (approximately 58 mg/100 g for individual phenolics and approximately 21430 mg/kg for individual amino acids). However, the total phenolic content, total flavonoid content and antioxidant activity values of DES extracts were determined between 59.09-77.50 mg GAE/100 g, 28.68-45.55 mg GAE/100 g and 42.96-146.86 mg TE/100 g, respectively. Therefore, it can be recommended to use these green solvents, which are known as environmentally friendly, as an alternative to organic solvents in the process of preparing extracts of this important medicinal plant in different areas.
在本研究中,旨在考察不同含量的深共熔溶剂(DESs)从龙蒿(Artemisia dracunculus L.)植物中提取生物活性化合物的能力。因此,对制备的DES提取物的总酚类-黄酮类含量、总原花青素含量以及抗氧化/抗菌活性进行了研究,同时还分析了单个酚类和单个氨基酸的谱图。结果表明,DES10在提取单个酚类(约59 mg/100 g)和单个氨基酸(约2500 mg/kg)方面效率最高,与乙醇(单个酚类约44 mg/100 g,单个氨基酸约19810 mg/kg)和甲醇(单个酚类约58 mg/100 g,单个氨基酸约21430 mg/kg)相比,其产率也更高。然而,DES提取物的总酚含量、总黄酮含量和抗氧化活性值分别在59.09 - 77.50 mg GAE/100 g、28.68 - 45.55 mg GAE/100 g和42.96 - 146.86 mg TE/100 g之间。因此,在不同领域制备这种重要药用植物提取物的过程中,推荐使用这些被认为是环境友好的绿色溶剂来替代有机溶剂。