Jørgensen L, Grasdalen H
Comp Biochem Physiol B. 1986;84(4):447-50. doi: 10.1016/0305-0491(86)90104-5.
31P-NMR was used to characterise intracellular phosphate pools and their post mortem changes at 7 degrees C in intact red and white cod muscles under anaerobic conditions. A total phosphate content of 55 and 60 mM was observed in red and white muscle, respectively. The concentration of P-creatine was 14 mM in the white and 9 mM in the red muscle, while that of inorganic phosphate, Pi (30 mM), ATP (9 mM), and sugar phosphate (5 mM) were similar in both muscles. During the first 90 min after death, the decrease in P-creatine showed a first order breakdown with a concomitant stoichiometric increase in Pi content, whereas the ATP and sugar phosphate remained the same. The intracellular pH decreased from 7.4 to 7.3 in this period. The steady-state rate constant of myosin ATPase was 0.0054 and 0.0022/min for red and white muscles, respectively. Individuals kept under diminished oxygen tension prior to being killed, showed a reduced P-creatine level in both muscles.
采用31P-核磁共振技术对完整的红鳕鱼和白鳕鱼肌肉在厌氧条件下于7摄氏度时细胞内磷酸盐池及其死后变化进行表征。红肌和白肌中的总磷酸盐含量分别为55 mM和60 mM。白肌中磷酸肌酸的浓度为14 mM，红肌中为9 mM，而无机磷酸盐（Pi，30 mM）、三磷酸腺苷（ATP，9 mM）和磷酸糖（5 mM）在两种肌肉中的浓度相似。死后最初90分钟内，磷酸肌酸的减少呈现一级分解，同时Pi含量化学计量增加，而ATP和磷酸糖保持不变。在此期间，细胞内pH从7.4降至7.3。红肌和白肌中肌球蛋白ATP酶的稳态速率常数分别为0.0054和0.0022/分钟。在处死前处于低氧张力下的个体，其两种肌肉中的磷酸肌酸水平均降低。
