Yoshizaki K
J Biochem. 1978 Jul;84(1):11-8. doi: 10.1093/oxfordjournals.jbchem.a132098.
31P-nuclear magnetic resonance was applied to living muscles of bullfrogs, and the time courses of metabolic changes of ATP, creatine phosphate, inorganic phosphate, and sugar phosphates were studied under anaerobic and aerobic conditions. A decrease in creatine phosphate was observed in the resting muscle under anaerobic conditions with a concomitant decrease in the intracellular pH, while the ATP level remained constant. With the use of 2,4-dinitro-1-fluorobenzene and iodoacetic acid, ATP disappeared quickly. When the resting muscle was perfused with oxygen-saturated glucose-Ringer's solution, the amount of creatine phosphate increased gradually. These findings indicate that anaerobic glycolysis is insufficient for even the resting energy consumption whereas oxidative phosphorylation is sufficient. The effects of tetanic stimulation on living muscles were also studied. When glycolysis and oxidative phosphorylation were suppressed, the intracellular energy store was depleted by the tetanic contraction. Anaerobic glycolysis produced rapid recovery of the energy store level, although it was insufficient to reach the initial level. Aerobic oxidative phosphorylation produced sufficient energy to reach the initial level, and this level was never exceeded. This finding suggests the existence of a regulatory mechanism for the energy store level.
将31P-核磁共振应用于牛蛙的活体肌肉，研究了在无氧和有氧条件下ATP、磷酸肌酸、无机磷酸和糖磷酸代谢变化的时间进程。在无氧条件下，静息肌肉中观察到磷酸肌酸减少，同时细胞内pH值下降，而ATP水平保持恒定。使用2,4-二硝基-1-氟苯和碘乙酸后，ATP迅速消失。当用氧饱和的葡萄糖-林格氏液灌注静息肌肉时，磷酸肌酸的量逐渐增加。这些发现表明，即使是静息能量消耗，无氧糖酵解也不足，而氧化磷酸化是足够的。还研究了强直刺激对活体肌肉的影响。当糖酵解和氧化磷酸化受到抑制时，强直收缩会耗尽细胞内的能量储备。无氧糖酵解使能量储备水平迅速恢复，尽管不足以恢复到初始水平。有氧氧化磷酸化产生足够的能量以恢复到初始水平，且该水平从未超过。这一发现表明存在能量储备水平的调节机制。
