College of Chemistry, Chemical Engineering and Materials Science, Zaozhuang University, Zaozhuang, Shandong, 277160, China.
State Key Laboratory of Elemento-organic Chemistry, Tianjin Key Laboratory of Biosensing and Molecular Recognition, College of Chemistry, Nankai University, Tianjin 300071, China.
Chem Commun (Camb). 2023 Aug 29;59(70):10552-10555. doi: 10.1039/d3cc03135g.
We report an effective assessment of lanthanide ion (Ln) delivery into live cells by paramagnetic NMR spectroscopy. Free Ln ions are toxic to live cells resulting in a gradual leakage of target proteins to the extracellular media. The citrate-Ln complex is an efficient and mild reagent over the free Ln form for live cell delivery.
我们报告了一种通过顺磁 NMR 光谱有效评估镧系离子（Ln）向活细胞内传递的方法。游离的 Ln 离子对活细胞有毒，导致靶蛋白逐渐漏出到细胞外培养基中。与游离的 Ln 形式相比，柠檬酸镧复合物是一种用于活细胞传递的有效且温和的试剂。
