通过顺磁 NMR 光谱法有效评估镧系元素离子向活细胞内的输送。

Effective assessment of lanthanide ion delivery into live cells by paramagnetic NMR spectroscopy.

机构信息

College of Chemistry, Chemical Engineering and Materials Science, Zaozhuang University, Zaozhuang, Shandong, 277160, China.

State Key Laboratory of Elemento-organic Chemistry, Tianjin Key Laboratory of Biosensing and Molecular Recognition, College of Chemistry, Nankai University, Tianjin 300071, China.

出版信息

Chem Commun (Camb). 2023 Aug 29;59(70):10552-10555. doi: 10.1039/d3cc03135g.

DOI:10.1039/d3cc03135g

PMID:37575089

Abstract

We report an effective assessment of lanthanide ion (Ln) delivery into live cells by paramagnetic NMR spectroscopy. Free Ln ions are toxic to live cells resulting in a gradual leakage of target proteins to the extracellular media. The citrate-Ln complex is an efficient and mild reagent over the free Ln form for live cell delivery.

摘要

我们报告了一种通过顺磁 NMR 光谱有效评估镧系离子（Ln）向活细胞内传递的方法。游离的 Ln 离子对活细胞有毒，导致靶蛋白逐渐漏出到细胞外培养基中。与游离的 Ln 形式相比，柠檬酸镧复合物是一种用于活细胞传递的有效且温和的试剂。