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在小鼠中使用光纤光度法估计睡眠期间荧光生物传感器水平。

Using Fiber Photometry in Mice to Estimate Fluorescent Biosensor Levels During Sleep.

作者信息

Andersen Mie, Tsopanidou Anastasia, Radovanovic Tessa, Compere Viviane Noelani, Hauglund Natalie, Nedergaard Maiken, Kjaerby Celia

机构信息

Center for Translational Neuromedicine, University of Copenhagen, Noerre Alle 14, 2200 Copenhagen, Denmark.

Center for Translational Neuromedicine, Department of Neurosurgery, University of Rochester Medical Center, Rochester, NY, USA.

出版信息

Bio Protoc. 2023 Aug 5;13(15):e4734. doi: 10.21769/BioProtoc.4734.

DOI:10.21769/BioProtoc.4734
PMID:37575397
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10415158/
Abstract

Sleep is not homogenous but contains a highly diverse microstructural composition influenced by neuromodulators. Prior methods used to measure neuromodulator levels in vivo have been limited by low time resolution or technical difficulties in achieving recordings in a freely moving setting, which is essential for natural sleep. In this protocol, we demonstrate the combination of electroencephalographic (EEG)/electromyographic (EMG) recordings with fiber photometric measurements of fluorescent biosensors for neuromodulators in freely moving mice. This allows for real-time assessment of extracellular neuromodulator levels during distinct phases of sleep with a high temporal resolution.

摘要

睡眠并非是均匀一致的，而是包含受神经调质影响的高度多样的微观结构组成。以往用于在体内测量神经调质水平的方法受到时间分辨率低或在自由活动状态下进行记录存在技术困难的限制，而自由活动状态对于自然睡眠至关重要。在本方案中，我们展示了将脑电图（EEG）/肌电图（EMG）记录与用于自由活动小鼠神经调质的荧光生物传感器的纤维光度测量相结合。这使得能够以高时间分辨率实时评估睡眠不同阶段细胞外神经调质水平。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d688/10415158/a763ab337f80/BioProtoc-13-15-4734-g011.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d688/10415158/816180aa5886/BioProtoc-13-15-4734-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d688/10415158/457f7a609f5c/BioProtoc-13-15-4734-v001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d688/10415158/b3c6164cae09/BioProtoc-13-15-4734-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d688/10415158/477b9e30070c/BioProtoc-13-15-4734-v002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d688/10415158/52e4641b087c/BioProtoc-13-15-4734-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d688/10415158/a763ab337f80/BioProtoc-13-15-4734-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d688/10415158/d12cad4b19e8/BioProtoc-13-15-4734-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d688/10415158/0253b4dc8bd3/BioProtoc-13-15-4734-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d688/10415158/1d582d97de49/BioProtoc-13-15-4734-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d688/10415158/2536a9e701af/BioProtoc-13-15-4734-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d688/10415158/ff3298455b83/BioProtoc-13-15-4734-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d688/10415158/53a588600838/BioProtoc-13-15-4734-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d688/10415158/3d3b271e8190/BioProtoc-13-15-4734-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d688/10415158/816180aa5886/BioProtoc-13-15-4734-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d688/10415158/457f7a609f5c/BioProtoc-13-15-4734-v001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d688/10415158/b3c6164cae09/BioProtoc-13-15-4734-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d688/10415158/477b9e30070c/BioProtoc-13-15-4734-v002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d688/10415158/52e4641b087c/BioProtoc-13-15-4734-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d688/10415158/a763ab337f80/BioProtoc-13-15-4734-g011.jpg

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2
Relating Pupil Diameter and Blinking to Cortical Activity and Hemodynamics across Arousal States.将瞳孔直径和眨眼与皮质活动和清醒状态下的血液动力学相关联。
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3
A noradrenergic-hypothalamic neural substrate for stress-induced sleep disturbances.
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Elife. 2025 Jan 30;13:RP98353. doi: 10.7554/eLife.98353.
4
Norepinephrine-mediated slow vasomotion drives glymphatic clearance during sleep.去甲肾上腺素介导的缓慢血管运动在睡眠期间驱动类淋巴系统清除。
Cell. 2025 Feb 6;188(3):606-622.e17. doi: 10.1016/j.cell.2024.11.027. Epub 2025 Jan 8.
5
Cold induces brain region-selective cell activity-dependent lipid metabolism.寒冷诱导脑区选择性的细胞活性依赖性脂质代谢。
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Neurovascular coupling and bilateral connectivity during NREM and REM sleep.非快速眼动睡眠和快速眼动睡眠期间的神经血管耦联和双侧连通性。
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