Department of Biomedical Engineering, The Pennsylvania State University, University Park, United States.
Center for Neural Engineering, The Pennsylvania State University, University Park, United States.
Elife. 2020 Oct 29;9:e62071. doi: 10.7554/eLife.62071.
To understand how arousal state impacts cerebral hemodynamics and neurovascular coupling, we monitored neural activity, behavior, and hemodynamic signals in un-anesthetized, head-fixed mice. Mice frequently fell asleep during imaging, and these sleep events were interspersed with periods of wake. During both NREM and REM sleep, mice showed large increases in cerebral blood volume ([HbT]) and arteriole diameter relative to the awake state, two to five times larger than those evoked by sensory stimulation. During NREM, the amplitude of bilateral low-frequency oscillations in [HbT] increased markedly, and coherency between neural activity and hemodynamic signals was higher than the awake resting and REM states. Bilateral correlations in neural activity and [HbT] were highest during NREM, and lowest in the awake state. Hemodynamic signals in the cortex are strongly modulated by arousal state, and changes during sleep are substantially larger than sensory-evoked responses.
为了了解觉醒状态如何影响大脑血液动力学和神经血管耦合，我们在未麻醉、头部固定的小鼠中监测神经活动、行为和血液动力学信号。在成像过程中，小鼠经常入睡，这些睡眠事件与清醒状态交替出现。在 NREM 和 REM 睡眠期间，与清醒状态相比，小鼠的大脑血容量 ([HbT]) 和小动脉直径显著增加，比感觉刺激引起的增加大两到五倍。在 NREM 期间，[HbT] 的低频振荡幅度明显增加，神经活动和血液动力学信号之间的相干性高于清醒休息和 REM 状态。在 NREM 期间，神经活动和 [HbT] 之间的双侧相关性最高，在清醒状态下最低。皮层的血液动力学信号受觉醒状态强烈调节，睡眠期间的变化比感觉诱发反应大得多。
