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可持续利用巨型芦苇生产工业化酶。

Sustainable use of giant reed to produce industrialized enzymes.

作者信息

Ascacio-Valdés A, De León-Medina J C, De León-Zapata M A, Laredo-Alcalá E I, Flores-Gallegos A C, Meléndez-Rentería N P

机构信息

Food Research Department, Faculty of Chemical Sciences, Autonomous University of Coahuila, Boulevard Venustiano Carranza without number, 25280, Saltillo, Mexico.

Research Center for the Conservation of Biodiversity and Ecology of Coahuila, Autonomous University of Coahuila, Hidalgo 212 central zone, 27540, Cuatrociénegas, Mexico.

出版信息

Heliyon. 2023 Jul 26;9(8):e18748. doi: 10.1016/j.heliyon.2023.e18748. eCollection 2023 Aug.

Abstract

The giant reed () is a fast-growing plant adapted to different climatic and soil conditions; although its origin is Asian, the species has spread throughout the world. During its development, it consumes three times more water than typical native vegetation and is responsible for changing the landscape of riparian areas; the high biomass productivity and the annual harvest period make this crop an alternative to produce and/or extract industrial bioproducts. The main objective of this research was to evaluate the feasibility of using giant reed in a bioprocess that produces enzymes by a solid-state fermentation experiment, four fungal species were tested ( GH1, PSH, , and ); enzyme activities were performed using reported methodologies varying only reaction volumes. The GH1 and PSH strains were the best adapted to the plant material, GH1 was capable to produce 4 of the 5 evaluated enzymes (cellulase-endoglucanase (174.39 ± 19.62 U/L), xylanase (1313.31 ± 39.25 U/L), invertase (642.22 ± 23.55 U/L), and polyphenol oxidase (6094.01 ± 306.54) while PSH was able to produce 3 of the 5 evaluated enzymes (cellulase-endoglucanase (147.09 ± 13.88 U/L), xylanase (1307.76 ± 31.40 U/L), and invertase (603.92 ± 3.14 U/L).

摘要

巨芦苇是一种生长迅速的植物,能适应不同的气候和土壤条件;尽管其原产于亚洲,但该物种已遍布全球。在其生长过程中,它消耗的水量是典型本土植被的三倍,并且导致河岸地区景观发生变化;高生物量生产力和年度收获期使这种作物成为生产和/或提取工业生物产品的一种选择。本研究的主要目的是通过固态发酵实验评估在生物过程中使用巨芦苇生产酶的可行性,测试了四种真菌菌株(GH1、PSH、 和 );酶活性采用已报道的方法进行测定,仅反应体积有所不同。GH1和PSH菌株对植物材料适应性最佳,GH1能够产生所评估的5种酶中的4种(纤维素酶 - 内切葡聚糖酶(174.39 ± 19.62 U/L)、木聚糖酶(1313.31 ± 39.25 U/L)、转化酶(642.22 ± 23.55 U/L)和多酚氧化酶(6094.01 ± 306.54)),而PSH能够产生所评估的5种酶中的3种(纤维素酶 - 内切葡聚糖酶(147.09 ± 13.88 U/L)、木聚糖酶(1307.76 ± 31.40 U/L)和转化酶(603.92 ± 3.14 U/L))。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/58ea/10412830/e348335bb4df/gr1.jpg

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