Chang Yu-Chung, Ge Xiaoxiao, Wang Li-Ju, Lee Stephen Sauchi, Paulsen Michael H, Khan Qaiser M, Khalid Zafar M, Bhalli Javed A, Waheed Usman, Simpson Christopher D, Du Dan, Li Lei, Lin Yuehe
School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, USA.
Department of Statistical Science, University of Idaho, Moscow, ID 83844, USA.
Sens Actuators B Chem. 2018 Dec 1;275:300-305. doi: 10.1016/j.snb.2018.08.009.
In this work, we present an ultra-low-cost smartphone device for in situ quantification of OP poisoning severity. The performance of the lens-less smartphone spectrum apparatus (LeSSA) is evaluated using standard human Interleukin-6 (IL-6) immunoassay kits. Upon dose-response curve fitting, LeSSA demonstrates an accuracy of 99.5%. The limit of detection (LOD) of LeSSA was evaluated through comparison of 6.4 pg/ml with standard laboratory grade UV-vis spectrophotometer at 5.5 pg/ml. Evaluating the capacity of LeSSA in spike solution by combining plasma cholinesterase (PChE) and human plasma shows consistency at agreement of 97.6% between LeSSA and the laboratory instrument. For application demonstration, the activity of PChE for 24 agricultural workers' plasma samples was measured with LeSSA, showing exceptional agreement (r2 = 0.92) with the laboratory instrument reference. In addition to near laboratory grade accuracy, the total manufacturing cost of LeSSA is only $20 USD highlighting it's affordability. With LeSSA, clinicians can evaluate OP poisoning severity without the need to transport patient samples to facilities at far distances. Utilizing LeSSA, immediate results can be used for administration of appropriate treatment.
在这项工作中，我们展示了一种用于现场定量有机磷中毒严重程度的超低成本智能手机设备。使用标准的人白细胞介素-6（IL-6）免疫分析试剂盒评估无透镜智能手机光谱仪（LeSSA）的性能。通过剂量反应曲线拟合，LeSSA的准确率为99.5%。通过将LeSSA的检测限（LOD）与标准实验室级紫外可见分光光度计在5.5 pg/ml时的6.4 pg/ml进行比较来评估。通过结合血浆胆碱酯酶（PChE）和人血浆评估LeSSA在加标溶液中的能力，结果显示LeSSA与实验室仪器之间的一致性为97.6%。为了进行应用演示，使用LeSSA测量了24名农业工人血浆样本中PChE的活性，结果显示与实验室仪器参考值具有极高的一致性（r2 = 0.92）。除了接近实验室级的准确性外，LeSSA的总制造成本仅为20美元，突出了其可承受性。有了LeSSA，临床医生无需将患者样本远距离运送到医疗机构就能评估有机磷中毒的严重程度。利用LeSSA，即时结果可用于指导适当的治疗。
