School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, United States; Key Laboratory of Luminescence and Real-Time Analytical Chemistry (Ministry of Education), College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China.
School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, United States; Key Laboratory of Jiangxi Province for Persistent Pollutants Control and Resources Recycle, College of Environmental and Chemical Engineering, Nanchang Hangkong University, Nanchang 330063, China.
Biosens Bioelectron. 2018 May 30;106:43-49. doi: 10.1016/j.bios.2018.01.033. Epub 2018 Jan 31.
Graphitic carbon nitride/bismuth ferrite nanocomposites (g-CN/BiFeO NCs) were synthesized by a facile one step sol-gel combustion method and employed as a peroxidase-like catalyst. Based on the catalytical activity on the luminol-HO reaction, the nanocomposites were utilized as a colorimetric/chemiluminescent dual-readout immunochromatographic assay (ICA) for the multiplexed detection of pesticide residues by utilizing chlorpyrifos and carbaryl as the model analytes. In the proposed protocol, chlorpyrifos antibody and carbaryl antibody were tagged to g-CN/BiFeO NCs for developing the spatially-resolved multianalyte ICA. After two competitive immunoreactions completed on the ICA test strip, the tracer antibodies were captured by the immobilized antigens on two test lines. The accumulation of g-CN/BiFeO NCs led to the appearance of brown color, which were observed as a colorimetric and semi-quantitative signal. Furthermore, the g-CN/BiFeO NCs-driven generation of CL signal was collected as a sensitively quantitative signal after initiating the luminol-HO reaction on the test lines. Under the optimal conditions, the limits of detection of chlorpyrifos and carbaryl were both 0.033ng/mL. The dual-readout ICA was successfully used to detect chlorpyrifos and carbaryl spiked in environmental water and traditional Chinese medicine samples with acceptable recovery values of 80-119% and 90-118%. Due to many advantages including low cost, time efficiency, high sensitivity and good portability, the novel ICA showed great potential in many areas such as drug safety, environmental monitoring and clinical diagnosis.
石墨相氮化碳/铋铁氧体纳米复合材料（g-CN/BiFeO NCs）通过简便的一步溶胶-凝胶燃烧法合成，并用作过氧化物酶样催化剂。基于对鲁米诺-HO 反应的催化活性，该纳米复合材料被用作比色/化学发光双读取免疫层析分析（ICA），用于利用毒死蜱和carbaryl 作为模型分析物进行农药残留的多重检测。在所提出的方案中，将毒死蜱抗体和carbaryl 抗体标记到 g-CN/BiFeO NCs 上，用于开发空间分辨多分析物 ICA。在 ICA 测试条上完成两次竞争性免疫反应后，示踪抗体被固定在两条测试线上的固定抗原捕获。g-CN/BiFeO NCs 的积累导致出现棕色，这被观察为比色和半定量信号。此外，在测试线上启动鲁米诺-HO 反应后，g-CN/BiFeO NCs 驱动的 CL 信号的产生被收集为敏感定量信号。在最佳条件下，毒死蜱和carbaryl 的检测限均为 0.033ng/mL。双读取 ICA 成功用于检测环境水和中药中添加的毒死蜱和 carbaryl，回收率在 80-119%和 90-118%之间。由于成本低、效率高、灵敏度高、便携性好等优点，新型 ICA 在药物安全、环境监测、临床诊断等领域具有广阔的应用前景。
