Arzumanyan Hranush, Avagyan Hranush, Voskanyan Henry, Simonyan Liana, Simonyan Jon, Semirjyan Zara, Karalyan Zaven
Laboratory of Cell Biology and Virology, Institute of Molecular Biology of NAS RA, Yerevan, Armenia.
Experimental Laboratory, Yerevan State Medical University, Yerevan, Armenia.
Vet World. 2023 May;16(5):1029-1034. doi: 10.14202/vetworld.2023.1029-1034. Epub 2023 May 15.
Recently, viral diseases of honey bees () have presented an increasing threat to beekeeping. This study aimed to examine the presence of honey bee viruses in and non- bee species, the mite , and pollinated plants in Armenia.
Sampling was performed in Tavush Province, in the northeast of the Republic of Armenia, from August to November 2019. Overall, 200 bees, 50 mites, and 20 wasps were collected (corresponding to three bees, five mites, and 2-11 wasps in each investigated sample) and homogenized for RNA isolation and detection of viruses. Ten pollinated plants were taken from each plant, and 2 g of each sample was used for homogenization. In each investigated case and plants received percentages of the virus presence.
Six important honey bee viruses (acute bee paralysis virus [ABPV], deformed wing virus [DWV], norovirus [ANV], Lake Sinai virus-2 [LSV-2], Big Sioux River virus [BSRV], and filamentous virus [AmFV]) were detected in samples by polymerase chain reaction. Our results showed that DWV, ANV, and ABPV were the most common viruses in honey bees. All viruses were detected in wasps, but LSV-2 and ANV were present in almost all samples.
Our results showed that almost all viruses were present in . Although ANV is very common in honey bees, it did not appear in any mite samples. Our study indicates that viruses typically associated with honey bees were also actively infecting wasps. Our data suggest that the survival of viruses in plants can be an important source of seasonal transmission of viruses to bees. In addition, pollinated plants can potentially serve as reservoirs for honey bee viruses.
近年来，蜜蜂的病毒性疾病对养蜂业构成了日益严重的威胁。本研究旨在检测亚美尼亚境内蜜蜂及非蜜蜂物种、狄斯瓦螨和授粉植物中蜜蜂病毒的存在情况。
2019年8月至11月在亚美尼亚共和国东北部的塔武什省进行采样。总共收集了200只蜜蜂、50只狄斯瓦螨和20只黄蜂（每个调查样本对应3只蜜蜂、5只狄斯瓦螨和2 - 11只黄蜂），并进行匀浆以分离RNA并检测病毒。从每种植物中选取10个授粉样本，每个样本取2克用于匀浆。在每个调查案例中，记录蜜蜂和植物的病毒感染率。
通过聚合酶链反应在样本中检测到六种重要的蜜蜂病毒（急性蜜蜂麻痹病毒[ABPV]、残翅病毒[DWV]、亚美尼亚诺如病毒[ANV]、西奈湖病毒2型[LSV - 2]、大苏族河病毒[BSRV]和亚美尼亚丝状病毒[AmFV]）。我们的结果表明，DWV、ANV和ABPV是蜜蜂中最常见的病毒。在黄蜂中检测到了所有病毒，但LSV - 2和ANV几乎存在于所有样本中。
我们的结果表明，几乎所有病毒都存在于蜜蜂中。虽然ANV在蜜蜂中非常常见，但在任何狄斯瓦螨样本中均未出现。我们的研究表明，通常与蜜蜂相关的病毒也在积极感染黄蜂。我们的数据表明，病毒在植物中的存活可能是病毒季节性传播给蜜蜂的重要来源。此外，授粉植物可能是蜜蜂病毒的潜在宿主。
