Chernukha Irina, Vasilevskaya Ekaterina, Klimina Ksenia, Yunes Roman, Kupaeva Nadezhda, Tolmacheva Galina, Kibitkina Anastasiya, Danilenko Valery, Karabanov Sergey, Fedulova Liliya
Department of Experimental Clinic and Research Laboratory for Bioactive Substances of Animal Origin, V.M. Gorbatov Federal Research Center for Food Systems, Moscow, Russia.
Department of Genetics of Microorganisms, Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia.
Vet World. 2023 May;16(5):929-938. doi: 10.14202/vetworld.2023.929-938. Epub 2023 May 7.
Prolonged stress causes deleterious effects on both the organism and its microbiota. In this study, we examined the effects of exposure to variable frequency ultrasound (US) on the gut microbiota-liver-brain axis of mice.
This study was conducted on 20 mature clinically healthy sexually naive C57BL/6J male mice (42-45 days old). Group 1 (Normal) consisted of healthy intact mice (n = 10). Group 2 (Stress) consisted of mice subjected to US-induced stress (n = 10) for 20 days with alternating frequencies (20-45 kHz). Stool samples were collected on days 0, 10, and 20, and the corresponding DNA was later subjected to 16SrRNA sequencing. After mice were sacrificed on day 21, the leukocyte count, blood serum biochemical parameters, and liver and brain antioxidant status were measured. Behavioral testing was performed on days 17, 18, and 19.
Ultrasound lead to higher stress and anxiety levels; increase in creatinine by 8.29% and gamma-glutamyltransferase activity by 5 times, a decrease in alkaline phosphatase activity by 38.23%, increase of de Ritis coefficient by 21.34%; increased liver and brain superoxide dismutase level by 20.8% and 21.5%, respectively; the stress-related changes in the gut microbiota composition - and .
Subjecting mice to 20 days of US-induced stress leads to systemic disorders due to oxidative stress and a decrease in the diversity of the gut microbiota.
长期应激会对机体及其微生物群产生有害影响。在本研究中，我们检测了暴露于可变频率超声对小鼠肠道微生物群-肝脏-脑轴的影响。
本研究选用20只成熟、临床健康、未接触过异性的C57BL/6J雄性小鼠（42 - 45日龄）。第1组（正常组）由健康未处理小鼠组成（n = 10）。第2组（应激组）由接受超声诱导应激的小鼠组成（n = 10），应激持续20天，频率交替变化（20 - 45千赫）。在第0、10和20天收集粪便样本，随后对相应的DNA进行16SrRNA测序。在第21天处死小鼠后，测量白细胞计数、血清生化参数以及肝脏和脑的抗氧化状态。在第17、18和19天进行行为测试。
超声导致应激和焦虑水平升高；肌酐增加8.29%，γ-谷氨酰转移酶活性增加至5倍，碱性磷酸酶活性降低38.23%，德瑞蒂斯系数增加21.34%；肝脏和脑中超氧化物歧化酶水平分别增加20.8%和21.5%；肠道微生物群组成出现与应激相关的变化——以及。
使小鼠接受20天超声诱导应激会导致由于氧化应激引起的全身紊乱以及肠道微生物群多样性降低。
