Stefan-van Staden Raluca-Ioana, Bratei Alexandru Adrian, Ilie-Mihai Ruxandra-Maria, Gheorghe Damaris-Cristina, Tuchiu Bianca Maria, Gurzu Simona
Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter 202 Splaiul Independentei Str. 060021 Bucharest-6 Romania
Faculty of Chemical Engineering and Biotechnologies, Politehnica University of Bucharest Bucharest Romania.
RSC Adv. 2023 Aug 11;13(34):24086-24092. doi: 10.1039/d3ra04260j. eCollection 2023 Aug 4.
Two miniaturized electrochemical devices were designed for the simultaneous bioanalysis of MMR (MLH1, MSH2, MSH6, PMS2), and of KRAS in whole blood, urine, saliva, and tumoral tissues. The devices comprised besides the electronic part of the potentiostat a combined 3D stochastic microsensor (combined microplatform) with the sensing part based on the modification of graphene decorated with nitrogen, sulfur and boron (NSB-EGR) modified with two types of frutafit: FTEX and FHD. For the assay of MSH2, MSH6, KRAS, and PMS2 higher sensitivities were recorded when the microdevice based on FHD was used, while for the assay of MLH1 the best sensitivity was achieved by using the microdevice based on FTEX. While the limits of quantification for MLH1, MSH2, and PMS2 were not influenced by the modifier, the microdevice based on FHD provided the lowest limit of quantification for KRAS, the microdevice based on FTEX provided the lowest limit of quantification for MSH6. The validation tests performed proved that recoveries of MLH1, MSH2, MSH6, PMS2, and of KRAS in whole blood, urine, saliva, and tumoral tissues higher than 98.50% with RSD (%) values lower than 0.10% were recorded.
设计了两种小型化电化学装置，用于同时对全血、尿液、唾液和肿瘤组织中的错配修复蛋白（MLH1、MSH2、MSH6、PMS2）和KRAS进行生物分析。这些装置除了包含恒电位仪的电子部分外，还包括一个组合式3D随机微传感器（组合微平台），其传感部分基于用两种类型的弗鲁塔菲特（FTEX和FHD）修饰的氮、硫和硼修饰的石墨烯（NSB-EGR）。在检测MSH2、MSH6、KRAS和PMS2时，使用基于FHD的微型装置记录到更高的灵敏度，而在检测MLH1时，使用基于FTEX的微型装置获得了最佳灵敏度。虽然MLH1、MSH2和PMS2的定量限不受修饰剂的影响，但基于FHD的微型装置对KRAS提供了最低的定量限，基于FTEX的微型装置对MSH6提供了最低的定量限。所进行的验证测试证明，全血、尿液、唾液和肿瘤组织中MLH1、MSH2、MSH6、PMS2和KRAS的回收率高于98.50%，相对标准偏差（RSD）（%）值低于0.10%。
