Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter, 202 Splaiul Independentei Str., 060021, Bucharest, Romania.
Faculty of Applied Chemistry and Material Science, Politehnica University of Bucharest, Bucharest, Romania.
Anal Bioanal Chem. 2020 May;412(13):3199-3207. doi: 10.1007/s00216-020-02575-y. Epub 2020 Mar 18.
Gastric cancer is the second leading cause of death in the world. Early detection will facilitate early treatment and full recovery of the patients. Metabolomics facilitated the detection of few amino acids able to be used as biomarkers. It was always assumed that the L-enantiomer of the chiral amino acid is part of the process, forgetting the twisting of DNA molecules which may also produce the D-enantiomer of the amino acid. Therefore, an enantioanalysis of amino acids such as glutamine which are part of the gastric cancer metabolomics is absolutely necessary. Four stochastic sensors based on immobilization of protoporphyrin IX, β-cyclodextrin, and 2,2-diphenyl-1-picrylhydrazyl on graphene materials were proposed for the enantioanalysis of glutamine in whole blood samples of patients with gastric cancer. Different signatures were obtained for the enantiomers for each stochastic sensor, making possible the enantioanalysis of glutamine in large concentration ranges-from fmol/L to mmol/L; these ranges facilitating the enantioanalysis of glutamine in healthy patients as well as in patients were found in early and later stages of gastric cancer. Graphical abstract.
胃癌是全球第二大致死原因。早期发现有助于早期治疗和患者的完全康复。代谢组学促进了少数氨基酸作为生物标志物的检测。人们一直认为手性氨基酸的 L-对映体是该过程的一部分，而忘记了 DNA 分子的扭曲也可能产生氨基酸的 D-对映体。因此，对参与胃癌代谢组学的谷氨酸等氨基酸进行对映体分析是绝对必要的。提出了基于原卟啉 IX、β-环糊精和 2,2-二苯基-1-苦基肼在石墨烯材料上固定化的四个随机传感器，用于对手性全血样品中的谷氨酸进行对映体分析。对于每个随机传感器，对映体获得了不同的特征，使得能够在从飞摩尔/升到毫摩尔/升的大浓度范围内对手性全血样品中的谷氨酸进行对映体分析；这些范围有助于对健康患者以及处于胃癌早期和晚期的患者的谷氨酸进行对映体分析。
