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用于组织工程应用的电纺聚乳酸/聚羟基辛酸酯PLA/P(3HO)支架的生物学和物理化学研究。

Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/P(3HO) scaffolds for tissue engineering applications.

作者信息

Solarz Daria, Witko Tomasz, Karcz Robert, Malagurski Ivana, Ponjavic Marijana, Levic Steva, Nesic Aleksandra, Guzik Maciej, Savic Sanja, Nikodinovic-Runic Jasmina

机构信息

Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University Lojasiewicza 11 30-348 Krakow Poland.

Jerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences Niezapominajek 8 30-239 Krakow Poland

出版信息

RSC Adv. 2023 Aug 11;13(34):24112-24128. doi: 10.1039/d3ra03021k. eCollection 2023 Aug 4.

DOI:10.1039/d3ra03021k
PMID:37577093
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10415749/
Abstract

Polyhydroxyoctanoate, as a biocompatible and biodegradable biopolymer, represents an ideal candidate for biomedical applications. However, physical properties make it unsuitable for electrospinning, currently the most widely used technique for fabrication of fibrous scaffolds. To overcome this, it was blended with polylactic acid and polymer blend fibrous biomaterials were produced by electrospinning. The obtained PLA/PHO fibers were cylindrical, smaller in size, more hydrophilic and had a higher degree of biopolymer crystallinity and more favorable mechanical properties in comparison to the pure PLA sample. Cytotoxicity evaluation with human lung fibroblasts (MRC5 cells) combined with confocal microscopy were used to visualize mouse embryonic fibroblasts (MEF 3T3 cell line) migration and distribution showed that PLA/PHO samples support exceptional cell adhesion and viability, indicating excellent biocompatibility. The obtained results suggest that PLA/PHO fibrous biomaterials can be potentially used as biocompatible, biomimetic scaffolds for tissue engineering applications.

摘要

聚羟基辛酸酯作为一种生物相容性和可生物降解的生物聚合物，是生物医学应用的理想候选材料。然而，其物理性质使其不适用于静电纺丝，而静电纺丝是目前制备纤维支架最广泛使用的技术。为克服这一问题，将其与聚乳酸共混，并通过静电纺丝制备了聚合物共混纤维生物材料。与纯聚乳酸样品相比，所得的聚乳酸/聚羟基辛酸酯纤维呈圆柱形，尺寸更小，更具亲水性，生物聚合物结晶度更高，机械性能更优。用人肺成纤维细胞（MRC5细胞）进行细胞毒性评估，并结合共聚焦显微镜观察小鼠胚胎成纤维细胞（MEF 3T3细胞系）的迁移和分布，结果表明聚乳酸/聚羟基辛酸酯样品支持优异的细胞黏附性和活力，表明其具有出色的生物相容性。所得结果表明，聚乳酸/聚羟基辛酸酯纤维生物材料有潜力用作组织工程应用中具有生物相容性的仿生支架。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da7a/10415749/43277d7c2053/d3ra03021k-f12.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da7a/10415749/5624fdfbbe5a/d3ra03021k-f5.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da7a/10415749/43277d7c2053/d3ra03021k-f12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da7a/10415749/d5de8babe8d1/d3ra03021k-f1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da7a/10415749/2d5b9c3eee45/d3ra03021k-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da7a/10415749/5624fdfbbe5a/d3ra03021k-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da7a/10415749/9b5dd522d2e9/d3ra03021k-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da7a/10415749/4a0b1384c062/d3ra03021k-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da7a/10415749/0e84ecd7364a/d3ra03021k-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da7a/10415749/914c27f1cc55/d3ra03021k-f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da7a/10415749/08ef250f93fc/d3ra03021k-f10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da7a/10415749/9591338ee555/d3ra03021k-f11.jpg
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