Mena-Porras Esteban, Contreras-Aleman Annaby, Guevara-Hidalgo María Francinie, Avendaño Soto Esteban, Batista Menezes Diego, Alvarez-Perez Marco Antonio, Chavarría-Bolaños Daniel
School of Dentistry, Universidad de Costa Rica, Ciudad Universitaria Rodrigo Facio, San Jose 11501-2060, Costa Rica.
Centro de Ingeniería y Ciencia de Materiales (CICIMA), Universidad de Costa Rica, Ciudad Universitaria Rodrigo Facio, San Jose 11501-2060, Costa Rica.
Pharmaceutics. 2025 Jan 14;17(1):106. doi: 10.3390/pharmaceutics17010106.
This study aimed to synthesize polylactic acid (PLA) nanofibrillar scaffolds loaded with ibuprofen (IBU) using electrospinning (ES) and air-jet spinning (AJS). The scaffolds were evaluated for their physicochemical properties, drug release profiles, and biocompatibility to assess their potential for local analgesic applications.
Solutions of 10% (/) PLA combined with IBU at concentrations of 10%, 20%, and 30% were processed into nanofibrillar membranes using ES and AJS. The scaffolds were characterized using scanning electron microscopy (SEM), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and Fourier-transformed infrared (FT-IR) spectroscopy. The drug release profile was assessed by ultraviolet-visible spectrophotometry (UV-Vis), and cell adhesion and viability were evaluated using fibroblast culture assays. Statistical analyses included qualitative analyses, -tests, and Likelihood ratio tests.
SEM revealed randomly arranged nanofibers forming reticulated meshes, with more uniform dimensions observed in the AJS group. TGA and DSC analyses confirmed the thermodynamic stability of the scaffolds and enthalpy changes consistent with IBU incorporation, which FT-IR and UV-Vis validated. Drug release was sustained over 384 h, showing no significant differences between ES and AJS scaffolds ( > 0.05). Cytotoxicity and cell viability assays confirmed scaffold biocompatibility, with cellular responses proportional to drug concentration but within safe limits.
PLA-IBU nanofibrillar scaffolds were successfully synthesized using ES and AJS. Both methods yielded biocompatible systems with stable properties and controlled drug release. Further, in vivo studies are necessary to confirm their clinical potential.
本研究旨在通过静电纺丝（ES）和气流纺丝（AJS）制备负载布洛芬（IBU）的聚乳酸（PLA）纳米纤维支架。对这些支架的物理化学性质、药物释放曲线和生物相容性进行评估，以评估其在局部镇痛应用中的潜力。
将10%（/）PLA与浓度为10%、20%和30%的IBU溶液通过ES和AJS加工成纳米纤维膜。使用扫描电子显微镜（SEM）、差示扫描量热法（DSC）、热重分析（TGA）和傅里叶变换红外（FT-IR）光谱对支架进行表征。通过紫外可见分光光度法（UV-Vis）评估药物释放曲线，并使用成纤维细胞培养试验评估细胞粘附和活力。统计分析包括定性分析、t检验和似然比检验。
SEM显示随机排列的纳米纤维形成网状网格，AJS组观察到的尺寸更均匀。TGA和DSC分析证实了支架的热稳定性以及与IBU掺入一致的焓变，FT-IR和UV-Vis验证了这一点。药物释放持续384小时，ES和AJS支架之间无显著差异（P>0.05）。细胞毒性和细胞活力试验证实了支架的生物相容性，细胞反应与药物浓度成正比，但在安全范围内。
使用ES和AJS成功合成了PLA-IBU纳米纤维支架。两种方法均产生了具有稳定性质和可控药物释放的生物相容性系统。此外，需要进行体内研究以确认其临床潜力。
