Mazumder Razib, Hussain Arif, Rahman Mohammad Mustafizur, Phelan Jody E, Campino Susana, Abdullah Ahmed, Clark Taane G, Mondal Dinesh
Laboratory Sciences and Services Division, International Centre for Diarrhoeal Disease Research Bangladesh (icddr,b), Dhaka, Bangladesh.
Department of Biotechnology and Genetic Engineering, Jahangirnagar University, Dhaka, Bangladesh.
Front Microbiol. 2023 Jul 27;14:1206757. doi: 10.3389/fmicb.2023.1206757. eCollection 2023.
Atypical forms exhibit unusual characteristics compared to typical strains. The HS-producing variants of some atypical strains cause a wide range of illnesses in humans and animals. However, there are sparse reports on such strains worldwide. We performed whole-genome sequencing (WGS) and detailed characterization of four HS-producing variants from poultry and human clinical sources in Dhaka, Bangladesh. All four isolates were confirmed as using biochemical tests and genomic analysis, and were multidrug-resistant (MDR). WGS analysis including an additional Chinese strain, revealed diverse STs among the five HS-producing genomes, with clonal complex ST10 being detected in 2 out of 5 genomes. The predominant phylogroup detected was group A ( = 4/5). The ( = 5/5) was the most predominant extended-spectrum beta-lactamase (ESBL) gene, followed by different alleles of ( -55,-65,-123; = 3/5). Multiple plasmid replicons were detected, with IncX being the most common. One strain was classified as enteropathogenic . The genomes of all five isolates harbored five primary and four secondary function genes related to HS production. These findings suggest the potential of these isolates to cause disease and spread antibiotic resistance. Therefore, such atypical forms should be included in differential diagnosis to understand the pathogenicity, antimicrobial resistance and evolution of HS-producing
与典型菌株相比，非典型菌株表现出不同寻常的特征。一些非典型菌株产生荚膜多糖（HS）的变体可在人和动物中引发多种疾病。然而，全球范围内关于此类菌株的报道稀少。我们对来自孟加拉国达卡家禽和人类临床样本的4株产生HS的变体进行了全基因组测序（WGS）和详细表征。通过生化试验和基因组分析确认，所有4株分离株均为多重耐药（MDR）。包括另外一株中国菌株在内的WGS分析显示，5个产生HS的基因组存在不同的序列类型（STs），5个基因组中有2个检测到克隆复合体ST10。检测到的主要系统发育群为A群（4/5）。最主要的超广谱β-内酰胺酶（ESBL）基因是blaCTX-M（5/5），其次是不同等位基因的blaTEM（-55、-65、-123；3/5）。检测到多个质粒复制子，其中IncX最为常见。1株菌株被分类为肠致病性大肠杆菌。所有5株分离株的基因组都含有5个与HS产生相关的主要功能基因和4个次要功能基因。这些发现表明这些分离株具有致病和传播抗生素耐药性的潜力。因此，在鉴别诊断中应纳入此类非典型菌株，以了解产生HS的菌株的致病性、抗菌耐药性和进化情况。
