Gray Mary C, Thomas Keena S, Lamb Evan R, Werner Lacie M, Connolly Kristie L, Jerse Ann E, Criss Alison K
bioRxiv. 2023 Aug 4:2023.08.03.551882. doi: 10.1101/2023.08.03.551882.
The bacterial pathogen is an urgent global health problem due to increasing numbers of infections, coupled with rampant antibiotic resistance. Vaccines against gonorrhea are being prioritized to combat drug-resistant Meningococcal serogroup B vaccines such as 4CMenB are predicted by epidemiology studies to cross-protect individuals from natural infection with and elicit antibodies that cross-react with Evaluation of vaccine candidates for gonorrhea requires a suite of assays for predicting efficacy in vitro and in animal models of infection, including the role of antibodies elicited by immunization. Here we present assays to evaluate antibody functionality after immunization: antibody binding to intact serum bactericidal activity, and opsonophagocytic killing activity using primary human neutrophils (polymorphonuclear leukocytes). These assays were developed with purified antibodies against and used to evaluate serum from mice that were vaccinated with 4CMenB or given alum as a negative control. Results from these assays will help prioritize gonorrhea vaccine candidates for advanced preclinical to early clinical study and will contribute to identifying correlates and mechanisms of immune protection against .
由于感染数量不断增加，再加上猖獗的抗生素耐药性，这种细菌性病原体成为一个紧迫的全球健康问题。对抗淋病的疫苗进行优先排序以对抗耐药性。流行病学研究预测，如4CMenB的B群脑膜炎球菌疫苗可交叉保护个体免受自然感染，并引发与[病原体名称未明确]交叉反应的抗体。评估淋病疫苗候选物需要一系列用于预测体外和动物感染模型中效力的试验，包括免疫引发的抗体的作用。在这里，我们展示了免疫后评估抗体功能的试验：抗体与完整[病原体名称未明确]的结合、血清杀菌活性以及使用原代人中性粒细胞（多形核白细胞）的调理吞噬杀伤活性。这些试验是用针对[病原体名称未明确]的纯化抗体开发的，并用于评估接种4CMenB或给予明矾作为阴性对照的小鼠的血清。这些试验的结果将有助于为淋病疫苗候选物从临床前晚期到临床早期研究确定优先级，并将有助于确定针对[病原体名称未明确]的免疫保护的相关因素和机制。
